Conductive Hydrogel Conduits with Growth Factor Gradients for Peripheral Nerve Repair in Diabetics with Non-Suture Tape
Conductive Hydrogel Conduits with Growth Factor Gradients for Peripheral Nerve Repair in Diabetics with Non-Suture Tape
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DOI：
10.1002/adhm.202200755
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发表时间：
2022-06-21
影响因子：
10
通讯作者：
Luo, Zhengtang
中科院分区：
文献类型：
--
作者：
Cai, Yuting;Huang, Qun;Luo, Zhengtang
糖尿病患者由于高血糖和微血管并发症而遭受周围神经损伤，并且再生缓慢且不完全。本研究开发了一种基于石墨烯的神经引导导管，通过将天然双网络水凝胶和神经营养浓度梯度结合起来，用于糖尿病患者的非侵入性治疗。GelMA/丝素蛋白双网络水凝胶具有快速凝固/固化、合适的机械支撑、良好的生物相容性和可持续的生长因子递送的四重作用。同时，石墨烯网可以改善管道的韧性，提高再生管道的导电性。在这里，新型丝带通过双重机制取代缝合步骤，显示出对湿组织的快速而坚韧的粘附。体内实验结果表明，在导管中梯度浓度的netrin-1比均匀浓度的netrin-1具有更好的性能，所述均匀浓度的netrin-1由趋化现象引起，以促进轴突延伸、髓鞘再生和血管生成。总之，GelMA/silk石墨烯导管与梯度netrin-1和干燥的双面胶带可以显著促进周围神经损伤的修复，抑制糖尿病患者肌肉萎缩。
Diabetic patients suffer from peripheral nerve injury with slow and incomplete regeneration owing to hyperglycemia and microvascular complications. This study develops a graphene-based nerve guidance conduit by incorporating natural double network hydrogel and a neurotrophic concentration gradient with non-invasive treatment for diabetics. GelMA/silk fibroin double network hydrogel plays quadruple roles for rapid setting/curing, suitable mechanical supporting, good biocompatibility, and sustainable growth factor delivery. Meanwhile, graphene mesh can improve the toughness of conduit and enhance conductivity of conduit for regeneration. Here, novel silk tapes show quick and tough adhesion of wet tissue by dual mechanism to replace suture step. The in vivo results demonstrate that gradient concentration of netrin-1 in conduit have better performance than uniform concentration caused by chemotaxis phenomenon for axon extension, remyelination, and angiogenesis. Altogether, GelMA/silk graphene conduit with gradient netrin-1 and dry double-sided adhesive tape can significantly promote repairing of peripheral nerve injury and inhibit the atrophy of muscles for diabetics.