A putative lipase gene EXTRA GLUME1 regulates both empty-glume fate and spikelet development in rice.
A putative lipase gene EXTRA GLUME1 regulates both empty-glume fate and spikelet development in rice.
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DOI：
10.1111/j.1365-313x.2008.03710.x
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发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Xue Y
中科院分区：
文献类型：
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作者：
Li H;Xue D;Gao Z;Yan M;Xu W;Xing Z;Huang D;Qian Q;Xue Y
最近的研究表明，内部花器官身份的分子控制似乎在单子叶植物和双子叶植物之间很大程度上是保守的，但很少有人知道单子叶植物外部花器官，一个独特的花结构在禾本科植物的发展的分子机制。在这项研究中，我们报告的水稻额外的葡萄糖1（EG1）基因，一个假定的脂肪酶基因，指定空颖命运和花分生组织的决定性的克隆。除了影响空颖片的身份和数量，突变EG1导致异位花器官形成在每个器官轮或额外的异位轮。在心皮的假定区域内形成了重复的颖片状结构或新的花器官原基，从而形成不确定的花分生组织。EG1在花序原基中表达较强，在发育中的花原基中表达较弱。我们还发现，花分生组织和器官的身份基因OsLHS 1表现出改变的表达模式和水平的eg1突变体，并可能是负责的多效性花缺陷在eg1。EG1是一种功能上不同于任何已知植物脂肪酶的III类脂肪酶，它揭示了一条调控水稻空颖命运和小穗发育的新途径。
Recent studies have shown that molecular control of inner floral organ identity appears to be largely conserved between monocots and dicots, but little is known regarding the molecular mechanism underlying development of the monocot outer floral organ, a unique floral structure in grasses. In this study, we report the cloning of the rice EXTRA GLUME1 (EG1) gene, a putative lipase gene that specifies empty-glume fate and floral meristem determinacy. In addition to affecting the identity and number of empty glumes, mutations in EG1 caused ectopic floral organs to be formed at each organ whorl or in extra ectopic whorls. Iterative glume-like structures or new floral organ primordia were formed in the presumptive region of the carpel, resulting in an indeterminate floral meristem. EG1 is expressed strongly in inflorescence primordia and weakly in developing floral primordia. We also found that the floral meristem and organ identity gene OsLHS1 showed altered expression with respect to both pattern and levels in the eg1 mutant, and is probably responsible for the pleiotropic floral defects in eg1. As a putative class III lipase that functionally differs from any known plant lipase, EG1 reveals a novel pathway that regulates rice empty-glume fate and spikelet development.