MULTIPLE SYSTEMS ORGAN FAILURE - MODULATION OF HEPATOCYTE PROTEIN-SYNTHESIS BY ENDOTOXIN ACTIVATED KUPFFER CELLS
MULTIPLE SYSTEMS ORGAN FAILURE - MODULATION OF HEPATOCYTE PROTEIN-SYNTHESIS BY ENDOTOXIN ACTIVATED KUPFFER CELLS
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DOI：
10.1097/00000658-198501000-00013
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发表时间：
1985-01-01
影响因子：
9
通讯作者：
SIMMONS, RL
中科院分区：
文献类型：
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作者：
KELLER, GA;WEST, MA;SIMMONS, RL
肝功能不全伴多系统器官衰竭（MSOF）综合征的病因尚不清楚。研究了巨噬细胞/枯否细胞介导的肝细胞功能调节可能在这种现象中发挥作用的可能性。培养分离的大鼠肝细胞，并通过3 H亮氨酸掺入蛋白质的cpm测量其蛋白质合成速率。将由35-40%枯否细胞组成的非实质大鼠肝细胞（NPC）加入肝细胞中，以建立含或不含内毒素的共培养物。单纯NPC和单纯内毒素均不影响肝细胞蛋白质合成。在内毒素存在的情况下，NPC引起肝细胞蛋白质合成的显著减少（4，396. ±. 449 cpm）与对照肝细胞（10，943. ±. 623 cpm）。显微镜形态学或排除台盼蓝的能力均未发生变化。内毒素刺激的枯否细胞制剂对肝细胞功能的调节可能部分代表了与MSOF综合征相关的肝功能不全的机制。
The etiology of hepatic insufficiency associated with the multiple systems organ failure (MSOF) syndrome is unclear. The possibility that a macrophage/Kupffer cell mediated modulation of hepatocyte function may play a role in this phenomenon was investigated. Isolated rat hepatocytes were cultured, and their rate of protein synthesis was measured by cpm of 3H leucine incorporation into protein. Nonparenchymal rat liver cells (NPC) comprised of 35-40% Kupffer cells were added to hepatocytes to establish a co-culture with or without endotoxin. Neither NPC alone nor endotoxin alone affected hepatocyte protein synthesis. In the presence of endotoxin, NPC caused a marked diminution of hepatocyte protein synthesis (4,396 .+-. 449 cpm) compared with control hepatocytes (10,943 .+-. 623 cpm). No charge in microscopic morphology or ability to exclude trypan blue occurred. This modulation of hepatocyte function by an endotoxin stimulated Kupffer cell preparation may, in part, represent the mechanism of hepatic insufficiency associated with the MSOF syndrome.