Prediction of accumulation of 131I-labelled meta-iodobenzylguanidine in neuroblastoma cell lines by means of reverse transcription and polymerase chain reaction.
Prediction of accumulation of 131I-labelled meta-iodobenzylguanidine in neuroblastoma cell lines by means of reverse transcription and polymerase chain reaction.
复制标题
通过逆转录和聚合酶链反应的神经母细胞瘤细胞系中131i标记的元二苯甲胺的积累预测。
DOI：
10.1038/bjc.1994.256
复制
发表时间：
1994-07
影响因子：
8.8
通讯作者：
Barrett, A
中科院分区：
文献类型：
--
作者：
Mairs, R J;Livingstone, A;Gaze, M N;Wheldon, T E;Barrett, A
放射性标记的间碘苄胍（mIBG）是目前神经母细胞瘤靶向放射治疗最有前途的选择之一。目前，除了根据诊断扫描的半定量推断（取决于肉眼可见肿瘤块的持续存在）外，还不存在用于预测个体患者肿瘤细胞中mIBG摄取的方法。一个生物学快速测定，可以应用在初始活检将是非常宝贵的选择患者的治疗策略，包括放射性标记的mIBG。我们已经评估了去甲肾上腺素转运蛋白基因的表达在六个人神经母细胞瘤细胞系和三个非神经嵴衍生的细胞系使用逆转录聚合酶链反应。在六个神经母细胞瘤细胞系中的五个中观察到该基因的转录，但在对照细胞中没有观察到该基因的转录。mIBG的基因表达与mIBG的活性细胞积累之间建立了高度显著的相关性（P <0.01）。有人建议，去甲肾上腺素转运蛋白基因转录本的半定量评价可以预测体内肿瘤的mIBG摄取。
Radiolabelled meta-iodobenzylguanidine (mIBG) currently provides one of the most promising options for targeted radiotherapy of neuroblastoma. No means currently exists for prediction of mIBG uptake in tumour cells of individual patients other than semiquantitative inferences from diagnostic scanning which depend on the continued existence of a macroscopic tumour mass. A biological rapid assay which could be applied at initial biopsy would be invaluable in selecting patients for therapeutic strategies which incorporate radiolabelled mIBG. We have assessed the expression of the noradrenaline transporter gene in six human neuroblastoma cell lines and in three non-neural crest-derived cell lines using reverse transcription followed by the polymerase chain reaction. Transcription of this gene was observed in five out of six neuroblastoma cell lines but in none of the control cells. A highly significant correlation was established (P < 0.01) between gene expression and active cellular accumulation of mIBG. It is suggested that semiquantitative evaluation of noradrenaline transporter gene transcripts may be predictive of mIBG uptake by tumours in vivo.