Glucocorticoid-inducible expression of a bacterial avirulence gene in transgenic Arabidopsis induces hypersensitive cell death
Glucocorticoid-inducible expression of a bacterial avirulence gene in transgenic Arabidopsis induces hypersensitive cell death
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DOI：
10.1046/j.1365-313x.1998.00106.x
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发表时间：
1998-04-01
期刊：
影响因子：
7.2
通讯作者：
Staskawicz, BJ
中科院分区：
文献类型：
--
作者：
McNellis, TW;Mudgett, MB;Staskawicz, BJ
丁香假单胞菌致病性菌株。在含有互补的RPS2抗病基因的拟南芥植物中，携带avrRpt2无毒基因的番茄特异性地诱导了超敏细胞死亡反应。在具有RPS2基因的拟南芥植物中，avrRpt2的瞬时表达已被证明可诱导超敏性细胞死亡。为了分析avrRpt2在拟南芥中条件表达的影响，在糖皮质激素诱导的严格调控启动子的控制下，构建了含有avrRpt2基因的转基因系。在含有RPS2基因的转基因细胞系中，地塞米松诱导avrRpt2的表达导致了特异性的超敏细胞死亡反应，类似于紫花假单胞菌诱导的超敏反应，并诱导了一种致病相关基因（PR1）的表达。有趣的是，在突变体rps2-101C背景下，avrRpt2的高水平表达导致植物胁迫并最终导致细胞死亡，这表明avrRpt2可能在丁香假单胞菌的毒力中起作用。含有糖皮质激素诱导的avrRpt2基因的转基因RPS2和RPS2植物将为抗性和易感植物中无毒基因特异性细胞死亡反应的遗传、生理、生化和分子解剖提供强大的新工具。
Pathogenic strains of Pseudomonas syringae pv. tomato carrying the avrRpt2 avirulence gene specifically induce a hypersensitive cell death response in Arabidopsis plants that contain the complementary RPS2 disease resistance gene. Transient expression of avrRpt2 in Arabidopsis plants having the RPS2 gene has been shown to induce hypersensitive cell death. In order to analyze the effects of conditional expression of avrRpt2 in Arabidopsis plants, transgenic lines were constructed that contained the avrRpt2 gene under the control of a tightly regulated, glucocorticoid-inducible promoter. Dexamethasone-induced expression of avrRpt2 in transgenic lines having the RPS2 gene resulted in a specific hypersensitive cell death response that resembled a Pseudomonas syringae-induced hypersensitive response and also induced the expression of a pathogenesis-related gene (PR1). Interestingly, high level expression of avrRpt2 in a mutant rps2-101C background resulted in plant stress and ultimately cell death, suggesting a possible role for avrRpt2 in Pseudomonas syringae virulence. Transgenic RPS2 and rps2 plants that contain the glucocorticoid-inducible avrRpt2 gene will provide a powerful new tool for the genetic, physiological, biochemical, and molecular dissection of an avirulence gene-specified cell death response in both resistant and susceptible plants.