Cell Surface-Anchored Fluorescent Aptamer Sensor Enables Imaging of Chemical Transmitter Dynamics
Cell Surface-Anchored Fluorescent Aptamer Sensor Enables Imaging of Chemical Transmitter Dynamics
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DOI：
10.1021/ja302551p
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发表时间：
2012-06-13
影响因子：
15
通讯作者：
Sando, Shinsuke
中科院分区：
文献类型：
--
作者：
Tokunaga, Takeshi;Namiki, Shigeyuki;Sando, Shinsuke
将荧光适体传感器应用于细胞外化学递质动力学的分析。我们利用生育酚标记的适体，其允许荧光适体直接锚定在细胞表面上，同时保留其作为荧光传感器的性能。将靶向腺嘌呤化合物的快速响应荧光DNA适体传感器锚定在脑星形胶质细胞表面。荧光成像的适体锚定的星形胶质细胞能够实时监测释放的腺嘌呤化合物作为神经胶质细胞递质，这是同步的钙波传播在邻近的细胞。
A fluorescent aptamer sensor was applied to the analysis of extracellular chemical transmitter dynamics. We utilized a tocopherol-labeled aptamer, which allowed the direct anchoring of the fluorescent aptamer on the cell surface while retaining its performance as a fluorescent sensor. The fast-responsive fluorescent DNA aptamer sensor, which targets adenine compounds, was anchored on the surface of brain astrocytes. Fluorescence imaging of the aptamer-anchored astrocytes enabled the real-time monitoring of release of adenine compounds as a gliotransmitter, which was synchronized with calcium wave propagation in neighboring cells.