Eleven phthalate esters and di(2-ethylhexyl) adipate in one-week duplicate diet samples obtained from hospitals and their estimated daily intake
Eleven phthalate esters and di(2-ethylhexyl) adipate in one-week duplicate diet samples obtained from hospitals and their estimated daily intake
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DOI：
10.1080/02652030010024474
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发表时间：
2001-05-01
期刊：
影响因子：
--
通讯作者：
Tonogai, Y
中科院分区：
文献类型：
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作者：
Tsumura, Y;Ishimitsu, S;Tonogai, Y
分析了1周内获得的一式两份饮食样品中的增塑剂，以估计每日摄入量。邻苯二甲酸酯如下：二乙基、二丙基、正丁基、二戊基、二己基、丁基苄基、二环己基、二（2-乙基己基）、二辛基、二异辛基（异构体的混合物）和二异壬基（混合物）。还测定了己二酸二（2-乙基己基）酯。用乙腈提取复合粉的均质化样品，通过萃取到正己烷中除去脂质，并使用Florisil（R）和Bondesil PSA（R）双层柱清洗乙腈层。邻苯二甲酸酯采用GC/MS（SIM）测定。通过该方法从强化食品混合物中回收邻苯二甲酸酯为62.5- 140.8%。由三个实验室评估的质量保证表明，在同一批次样品中检测到的邻苯二甲酸酯水平的变异系数低于10%。每种邻苯二甲酸酯的检测限为0.1-23 ng/g。一个星期的重复饮食样本提供的三家医院在三个偏远的县，日本进行了分析，作为个人餐。在所有63个样本中，DEHP的含量在所有邻苯二甲酸盐中最高，范围为10-4400 ng/g。所有样本中估计的增塑剂摄入量为519 mug DEHP/天、86 mug DEHA/天、65 mug DINP/天和4.7 mug BBP/天。对于体重为50 kg的人（每天1850 mug），21天中有2天样本中计算的DEHP超过EU TDI。当局怀疑在制备膳食时使用的一次性聚氯乙烯手套是高DEHP含量的来源。在所有情况下，其他邻苯二甲酸酯和DEHA的单日摄入量均低于TDI的7%。在质量保证工作中使用的婴儿食品中发现高浓度的DEHP（5990 ng/g）。污染源是生产过程中使用的PVC管，通过用不锈钢管替换PVC管，有效减少了污染。
Plasticizers in duplicate diet samples obtained over 1 week were analysed in order to estimate daily intake. The phthalate esters were as follows: diethyl, dipropyl, dibutyl, dipentyl, dihexyl, butylbenzyl, dicyclohexyl, di( 2-ethylhexyl), dioctyl, diisooctyl (mixture of isomers) and diisononyl (mixture). Di( 2-ethylhexyl) adipate was also determined. Homogenized samples of composite meals were extracted with acetonitrile, lipids were removed by extraction into n-hexane and the acetonitrile layer was cleaned using Florisil(R) and Bondesil PSA(R) dual layer column. Phthalates were determined by GC/MS (SIM). Phthalate recovery from the fortified food mixture by this method was 62.5-140.8%. Quality assurance as assessed by three laboratories indicated coefficient of variance in the levels of detected phthalates in same lot samples as below 10%. Detection limits were 0.1-23 ng/g for each phthalate. One-week duplicate diet samples provided by three hospitals in three remote prefectures of Japan were analysed as individual meals. In all 63 samples, DEHP was present at the highest level among all phthalates in the range 10-4400 ng/g. The intake of plasticizers estimated from all samples was 519 mug DEHP/day, 86 mug DEHA/day, 65 mug DINP/day, and 4.7 mug BBP/day. Calculated DEHP in 2-day samples out of 21 days exceeded EU TDI for a person of 50 kg body weight (1850 mug per day). Disposable PVC gloves used during the preparation of meals were suspected as the source of the high DEHP content. One-day intake of the other phthalates and DEHA was below 7% of TDI in all cases. High concentrations of DEHP (5990 ng/g) was found in baby food used in quality assurance work. The source of contamination was the PVC-tube used during production and was effectively reduced by replacing the tube by one made of stainless steel.