Universal DNA biosensing based on instantaneously electrostatic attraction between hexaammineruthenium (III) and DNA molecules
Universal DNA biosensing based on instantaneously electrostatic attraction between hexaammineruthenium (III) and DNA molecules
复制标题
基于六氨钌 (III) 和 DNA 分子之间瞬时静电引力的通用 DNA 生物传感
DOI：
10.1016/j.bios.2018.12.019
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发表时间：
2019-02-15
影响因子：
12.6
通讯作者：
Yao, Bo
中科院分区：
文献类型：
--
作者：
Dai, Siya;Lu, Wei;Yao, Bo
尽管DNA生物传感器通过采用各种材料和技术取得了快速进展，但大多数报道的传感器都是基于对DNA片段的特定识别，而不能对DNA分子（即基因组DNA）进行通用测量。在这项工作中，我们提出了一种新的DNA生物传感方法，基于瞬时静电吸引（IEA）的六胺矿铵（III）和DNA分子。自由扩散的Ru(NH3)(6)（3+）由于其与磷酸盐骨架快速而强的静态相互作用而引起的电流变化被用作检测溶液中DNA分子的通用探针，而无需将捕获探针固定在电极上。优化后，将300 μ M Ru(NH3)(6)（3+）溶液中的30 μ L加入到工作电极直径为2 mm的金电极上，检测限为3.8 ng/ μ L，相当于DNA定量常用仪器NanoDror One。该方法使用可重复使用且价格低廉的金电极，提供了一种易于操作的序列无关DNA检测方法，并被证明能够直接检测基因组和质粒DNA。
Despite rapid progress in DNA biosensors by employing various materials as well as techniques, most of the reported sensors are based on specific recognition of a DNA fragment, however can not perform universal measurement of DNA molecules (i.e. genomic DNA). In this work, we proposed a novel DNA biosensing method based on instantaneously electrostatic attraction (IEA) between hexaammineruthenium (III) and DNA molecules. The current variation of freely diffused Ru(NH3)(6)(3+) caused by its quick and strong static interaction with phosphate backbones was employed as a universal probe to detect DNA molecules in solution, with no need for immobilization of capture probes on the electrode. After optimization, 30 mu L of 300 mu M Ru(NH3)(6)(3+) solution was added onto the gold electrode with a working electrode diameter of 2 mm, and a detection limit of 3.8 ng/mu L. was achieved, which is equivalent to NanoDror One spectrometer, the commonly used instrument for DNA quantification. Using reusable and inexpensive gold electrode, the approach provided an easy-operated sequence-independent DNA detection method, and was proved to be able to detect genomic and plasmid DNA directly.