Kinetic modeling and sensitivity analysis of acetone-butanol-ethanol production
Kinetic modeling and sensitivity analysis of acetone-butanol-ethanol production
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DOI：
10.1016/j.jbiotec.2007.05.005
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发表时间：
2007-08-01
影响因子：
4.1
通讯作者：
Sonomoto, Kenji
中科院分区：
文献类型：
--
作者：
Shinto, Hideaki;Tashiro, Yukihiro;Sonomoto, Kenji
利用新型模拟软件WinBEST-KIT建立了一个代谢途径的动力学模拟模型，该模型描述了Clostridium saccharoperbutylacetonicum N1-4生产丙酮-丁醇-乙醇（ABE）过程中代谢物的动态行为。通过与批培养中代谢物在大范围初始葡萄糖浓度（36.1-295 mM）下的实验时间过程数据进行比较，验证了该模型。通过引入底物抑制、丁醇产物抑制、丁酸活化，并考虑葡萄糖耗竭后能量不足情况下代谢反应的停止，修正模型中代谢产物实验时程与计算时程的平方相关系数（r(2)）为0.901。因此，最终修正的模型被认为是描述ABE生产中代谢物动态行为的动力学模拟的最佳候选模型之一。敏感性分析表明，丁酸酯逆反应途径（R-17）反应增加5%，丁酸酯辅酶a转移酶（R-15）反应减少5%是丁醇高产的重要因素。这些系统分析将有助于阐明丁醇高产的代谢瓶颈。(c) 2007 Elsevier B.V.版权所有
A kinetic simulation model of metabolic pathways that describes the dynamic behaviors of metabolites in acetone-butanol-ethanol (ABE) production by Clostridium saccharoperbutylacetonicum N1-4 was proposed using a novel simulator WinBEST-KIT. This model was validated by comparing with experimental time-course data of metabolites in batch cultures over a wide range of initial glucose concentrations (36.1-295 mM). By introducing substrate inhibition, product inhibition of butanol, activation of butyrate and considering the cessation of metabolic reactions in the case of insufficiency of energy after glucose exhaustion, the revised model showed 0.901 of squared correlation coefficient (r(2)) between experimental time-course of metabolites and calculated ones. Thus, the final revised model is assumed to be one of the best candidates for kinetic simulation describing dynamic behavior of metabolites in ABE production. Sensitivity analysis revealed that 5% increase in reaction of reverse pathway of butyrate production (R-17) and 5% decrease in reaction of CoA transferase for butyrate (R-15) highly contribute to high production of butanol. These system analyses should be effective in the elucidation which pathway is metabolic bottleneck for high production of butanol. (c) 2007 Elsevier B.V. All rights reserved.