Engineering designer nucleases with customized cleavage specificities.
Engineering designer nucleases with customized cleavage specificities.
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DOI：
10.1002/0471142727.mb1213s96
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发表时间：
2011-10
影响因子：
--
通讯作者：
Joung, J Keith
中科院分区：
文献类型：
--
作者：
Sander, Jeffry D;Maeder, Morgan L;Joung, J Keith
工程化的设计核酸酶可用于在多种模式生物和细胞类型中有效地修饰基因组序列。锌指核酸酶（ZFN）由融合到非特异性切割结构域的工程化锌指阵列组成，已被广泛用于修饰广泛的内源基因。在这里，我们描述了使用上下文相关组装（CoDA）方法工程化ZFN靶向特定基因序列的协议。
Engineered designer nucleases can be used to efficiently modify genomic sequence in a wide variety of model organisms and cell types. Zinc finger nucleases (ZFNs), consisting of an engineered zinc finger array fused to a non-specific cleavage domain, have been extensively used to modify a broad range of endogenous genes. Here we describe protocols for engineering ZFNs targeted to specific gene sequences of interest using the Context-Dependent Assembly (CoDA) method.