A Comparative Study of MH and EMS in the Induction of Chromosomal Aberrations on Lateral Root Meristem in Clitoria ternatea L
A Comparative Study of MH and EMS in the Induction of Chromosomal Aberrations on Lateral Root Meristem in Clitoria ternatea L
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DOI：
10.1508/cytologia.57.259
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发表时间：
1992-06
期刊：
影响因子：
1
通讯作者：
B. C. Patil;G. Bhat
中科院分区：
文献类型：
--
作者：
B. C. Patil;G. Bhat
研究了生长延缓剂(MH)和化学增效剂EMS(化学增效剂)对黄连幼苗侧根有丝分裂行为的相对影响。蓝色单人间。用MH(10~(-4)M、10~(-3)M和10~(-2)M)、EMS(0.15%、0.2%、0.25%)处理根2小时。在不同的恢复期(12小时、24小时、48小时、72小时)进行有丝分裂分析，以评估细胞学异常。对照组记录到2.68%的染色体畸变率。在两种处理中，观察到的常见异常类型是粘连、桥接、后期和末期滞后。在EMS治疗的情况下，研究表明染色体损伤，但不影响有丝分裂抑制，而MH在较高浓度(10-2M)和恢复期的所有时间因素对细胞分裂都表现出抑制作用，导致生长迟缓，相反，在较低浓度(10-4M)不同恢复期的情况下，没有丝裂分裂抑制，但细胞表现出与EMS一样良好的染色体畸变率，传递其诱变活性。
Relative effects of MH (Growth retardant) and EMS, (Chemical multagen) with respect to mitotic behaviour has been assessed on lateral roots of the seedlings of C. tennatea Var. blue single. Roots have been treated with MH (10-4M, 10-3M and 10-2M), EMS (0.15%, 0.2%, 0.25%) for 2hr. After different recovery periods, (12 hr, 24 hr, 48 hr, 72 hr) mitotic analysis were carried out to estimate the cytological anomalies. In control 2.68% of chromosomal aberrations have been recorded. In both the treatments, common type of abnormalities observed were stickiness, bridges, laggards at anaphase and telophase. In case of EMS treatment study indicates chromosomal damage, however, it does not affect the mitotic inhibition, whereas, MH at higher concentration (10-2M) and all time factors of recovery period exhibits inhibition action on cell division leading to growth retardation, on the contrary, at lower concentration (10-4M) with various recovery period there is no mitotic inhibition but cells show good percentage of chromosomal aberrations conveying its mutagenic activity like EMS.