The role of NGF in pregnancy-induced degeneration and regeneration of sympathetic nerves in the guinea pig uterus
The role of NGF in pregnancy-induced degeneration and regeneration of sympathetic nerves in the guinea pig uterus
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DOI：
10.1016/s0165-1838(99)00094-6
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发表时间：
2000-02-14
期刊：
影响因子：
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通讯作者：
Crutcher, KA
中科院分区：
文献类型：
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作者：
Brauer, MM;Shockley, KP;Crutcher, KA
在豚鼠中，怀孕与子宫交感神经中去甲肾上腺素的全面枯竭有关，在胎儿周围的子宫区域，交感神经纤维完全退化。这些妊娠引起的变化被解释为胎盘激素对颈旁神经节产生的交感神经短纤维系统的选择性影响。另一种解释是，怀孕会影响子宫的神经营养能力。我们用双点酶联免疫吸附试验检测了早孕、晚孕和产后早期豚鼠子宫下端、输卵管末端和宫颈中NGF蛋白的水平。为便于比较，用组织化学方法测定含去甲肾上腺素的交感神经纤维的分布和相对密度，用高效液相色谱-电化学检测法测定组织去甲肾上腺素水平。在分析的所有子宫区域中，NGF蛋白水平在足月妊娠时显示下降，但在任何情况下这种变化在统计学上都不显著。分娩后，宫颈以及可育和空角中的NGF蛋白水平均显著增加。这些结果表明，NGF蛋白的改变不能解释妊娠期子宫交感神经支配的损害，但可能有助于产后恢复。(C)2000由爱思唯尔科学公司出版。保留所有权利。
In the guinea pig, pregnancy is associated with a generalised depletion of noradrenaline in uterine sympathetic nerves and, in the areas of the uterus surrounding the foetus, by a complete degeneration of sympathetic nerve fibres. These pregnancy-induced changes have been interpreted as a selective effect of placental hormones on the system of short sympathetic fibres arising from the paracervical ganglia. An alternative explanation is that pregnancy affects the neurotrophic capacity of the uterus. We measured NGF-protein levels in the guinea pig uterine hem, tubal end and cervix at early pregnancy, late pregnancy and early postpartum, using a two-site enzyme-linked immunosorbent assay. For comparative purposes the distribution and relative density of noradrenaline-containing sympathetic nerve fibres were assessed histochemically, and tissue levels of noradrenaline were measured biochemically, using high-performance liquid chromatography with electrochemical detection. In all the uterine regions analysed, NGF-protein levels showed a decline at term pregnancy, but in no case was this change statistically significant. After delivery, NGF-protein levels showed a marked increase in the cervix as well as in both the fertile and empty horns. These results suggest that alterations in NGF-protein do not account for the impairment of uterine sympathetic innervation during pregnancy, but may contribute to their recovery after delivery. (C) 2000 Published by Elsevier Science B.V. All rights reserved.