The outer membrane localization of the Neisseria gonorrhoeae MsrA/B is involved in survival against reactive oxygen species
The outer membrane localization of the Neisseria gonorrhoeae MsrA/B is involved in survival against reactive oxygen species
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DOI：
10.1073/pnas.152334799
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发表时间：
2002-07-23
影响因子：
11.1
通讯作者：
Seifert, HS
中科院分区：
文献类型：
--
作者：
Skaar, EP;Tobiason, DM;Seifert, HS
据报道，淋病奈瑟菌的 PilB 蛋白参与菌毛蛋白基因转录的调节，但它也与肽蛋氨酸亚砜还原酶家族，特别是大肠杆菌的 MsrA 和 MsrB 具有显着的同源性。大肠杆菌中的 MsrA 和 MsrB 能够将蛋白质中的蛋氨酸亚砜残基还原为蛋氨酸。此外，淋球菌 PilB 蛋白编码与蛋白质氧化损伤修复相关的 MsrA 和 MsrB 活性。在这项工作中，我们证明淋病奈瑟菌的 PilB 蛋白不参与菌毛表达。此外，我们发现野生型淋病奈瑟菌产生两种形式的这种多肽，其中一种含有信号序列，从细菌细胞质分泌到外膜；另一种含有信号序列，从细菌细胞质分泌到外膜。另一个缺乏信号序列并且是细胞质的。此外，我们发现 PilB 蛋白的分泌形式与氧化损伤存在下的生存有关。
The PilB protein of Neisseria gonorrhoeae has been reported to be involved in the regulation of pilin gene transcription, but it also possesses significant homology to the peptide methionine sulfoxide reductase family of enzymes, specifically MsrA and MsrB from Escherichia coli. MsrA and MsrB in E. coli are able to reduce methionine sulfoxide residues in proteins to methionines. In addition, the gonococcal PilB protein encodes for both MsrA and MsrB activity associated with the repair of oxidative damage to proteins. In this work, we demonstrate that the PilB protein of Neisseria gonorrhoeae is not involved in pilus expression. Additionally, we show that wild-type N. gonorrhoeae produces two forms of this polypeptide, one of which contains a signal sequence and is secreted from the bacterial cytoplasm to the outer membrane; the other lacks a signal sequence and is cytoplasmic. Furthermore, we show that the secreted form of the PilB protein is involved in survival in the presence of oxidative damage.