Asymmetric Ketone Reduction by Immobilized Rhodotorula mucilaginosa
Asymmetric Ketone Reduction by Immobilized Rhodotorula mucilaginosa
复制标题
固定化粘红酵母的不对称酮还原
DOI：
10.3390/catal8040165
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发表时间：
2018-04-01
期刊：
影响因子：
3.9
通讯作者：
Chen, Bi-Shuang
中科院分区：
文献类型：
--
作者：
Liu, Hui;Duan, Wen-Di;Chen, Bi-Shuang
在我们之前的研究中，通过高通量筛选，筛选出粘红酵母(Rhodotorula稠ilaginosa)作为对酮的不对称还原反应具有非常高活性和选择性的全细胞生物催化剂。在这项研究中，该菌株的固定化细胞将酮还原为所需的手性醇。傅立叶变换红外光谱(FTIR)和扫描电子显微镜(SEM)表征表明，琼脂、海藻酸钙、PVA-海藻酸盐和壳聚糖组成的载体成功地固定了整个粘质红曲霉细胞。将固定化细胞用于14种不同芳香酮的对映选择性还原。琼脂和海藻酸钙固定化粘质红假单胞菌的效果较好。以琼脂为载体的固定化细胞与游离细胞相比，在pH 3.5~9范围内具有较高的pH耐受性和较高的热稳定性。固定在琼脂上的细胞在4℃下保存60d后仍保持90%的活性，6次重复使用后仍保持近100%的活性。此外，固定化程序非常简单，造成的污染最小。这些结果表明，固定化粘菌在生产手性醇的工业规模上是可行的。
In our previous study, Rhodotorula mucilaginosa (R. mucilaginosa) was selected via high throughput screening as a very active and selective whole-cell biocatalyst for the asymmetric reduction of ketones. In this study, the reduction of ketones to the desired chiral alcohols by immobilized cells of this strain was investigated. Characterization with Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) showed that whole R. mucilaginosa cells were successfully immobilized on support matrices composed of agar, calcium alginate, PVA-alginate and chitosan. The immobilized cells were applied to the enantioselective reduction of fourteen different aromatic ketones. Good to excellent results were achieved with R. mucilaginosa cells immobilized on agar and calcium alginate. The immobilized cells on the selected support matrix composed of agar exhibited a significant increase in pH tolerance at pH 3.5-9 and demonstrated highly improved thermal stability compared to free cells. The cells immobilized on agar retained 90% activity after 60 days storage at 4 degrees C and retained almost 100% activity after 6 reuse cycles. In addition, the immobilization procedures are very simple and cause minimal pollution. These results suggest that the application of immobilized R. mucilaginosa can be practical on an industrial scale to produce chiral alcohols.