Volatiles of bacterial antagonists inhibit mycelial growth of the plant pathogen Rhizoctonia solani
Volatiles of bacterial antagonists inhibit mycelial growth of the plant pathogen Rhizoctonia solani
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DOI：
10.1007/s00203-006-0199-0
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发表时间：
2007-05-01
影响因子：
2.8
通讯作者：
Piechulla, Birgit
中科院分区：
文献类型：
--
作者：
Kai, Marco;Effmert, Uta;Piechulla, Birgit
细菌拮抗剂是对其他生物体生长产生负面影响的细菌。许多拮抗剂通过多种机制抑制真菌的生长，例如溶解酶、铁载体和抗生素的分泌。这种对真菌生长的抑制可以间接支持植物生长。在这里，我们证明细菌拮抗剂释放的小有机挥发性化合物（VOC）会对土传植物病原真菌立枯丝核菌（Rhizoctonia solani Kuhn）的菌丝生长产生负面影响。在测试条件下，观察到嗜麦芽寡养单胞菌 R3089、普城沙雷氏菌 HRO-C48、根际寡养单胞菌 P69、臭味沙雷氏菌 4Rx13、普通假单胞菌 3Re2-7、普城沙雷氏菌 3Re4-18 和芽孢杆菌具有强抑制作用 (99-80%) 枯草芽孢杆菌B2g。荧光假单胞菌 L13-6-12 和洋葱伯克霍尔德菌 1S18 实现了 30% 的生长减少。通过顶空收集和 GC-MS 分析获得的这些拮抗剂的 VOC 谱显示出不同的成分和复杂性，范围从 1 种到近 30 种化合物不等。大多数挥发物是物种特异性的，但沙雷氏菌属的挥发物模式存在重叠。和假单胞菌属。由于与数据库中挥发物的质谱不匹配，许多细菌挥发性有机化合物无法被识别。
Bacterial antagonists are bacteria that negatively affect the growth of other organisms. Many antagonists inhibit the growth of fungi by various mechanisms, e.g., secretion of lytic enzymes, siderophores and antibiotics. Such inhibition of fungal growth may indirectly support plant growth. Here, we demonstrate that small organic volatile compounds (VOCs) emitted from bacterial antagonists negatively influence the mycelial growth of the soil-borne phytopathogenic fungus Rhizoctonia solani Kuhn. Strong inhibitions (99-80%) under the test conditions were observed with Stenotrophomonas maltophilia R3089, Serratia plymuthica HRO-C48, Stenotrophomonas rhizophila P69, Serratia odorifera 4Rx13, Pseudomonas trivialis 3Re2-7, S. plymuthica 3Re4-18 and Bacillus subtilis B2g. Pseudomonas fluorescens L13-6-12 and Burkholderia cepacia 1S18 achieved 30% growth reduction. The VOC profiles of these antagonists, obtained through headspace collection and analysis on GC-MS, show different compositions and complexities ranging from 1 to almost 30 compounds. Most volatiles are species-specific, but overlapping volatile patterns were found for Serratia spp. and Pseudomonas spp. Many of the bacterial VOCs could not be identified for lack of match with mass-spectra of volatiles in the databases.