Ovarian development and insulin-signaling pathways during reproductive differentiation in the queenless ponerine ant Diacamma sp.
Ovarian development and insulin-signaling pathways during reproductive differentiation in the queenless ponerine ant Diacamma sp.
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DOI：
10.1016/j.jinsphys.2009.10.013
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发表时间：
2010-03-01
影响因子：
2.2
通讯作者：
Miura, Toru
中科院分区：
文献类型：
--
作者：
Okada, Yasukazu;Miyazaki, Satoshi;Miura, Toru
在许多社会性hyperteran物种，工人拥有功能卵巢是生理上不活跃的女王的存在。我们研究了一种无蜂王的雌蚁Diacamma sp.的卵巢调节机制，使用组织学和分子技术。在这种蚂蚁中，明确的生殖分化发生通过一个高度复杂的优势行为相互作用称为“gemmae切割”。生殖生理学的这一清晰而快速的分支使我们能够阐明详细的卵巢分化过程。功能卵巢（梭体和环管）的组织学特征被发现在工人和繁殖，这表明早期卵子发生在工人中没有被阻止。由于已知胰岛素/胰岛素样生长因子信号传导（IIS）控制昆虫生殖，因此在Diacamma中克隆了2个正IIS调节因子胰岛素受体和丝氨酸-苏氨酸激酶Akt（蛋白激酶B）的直向同源物（DiaInR，DiaAkt）;通过实时定量聚合酶链反应检测了它们在生殖分化过程中的表达模式; DiaInR和DiaAkt在生殖动物的胃中强烈表达。整体原位杂交结果表明，DiaInR和DiaAkt在繁殖动物的滋养细胞、卵母细胞和上生殖区表达，而在工蚁中不表达。我们的数据表明，IIS途径解释了卵子发生后期的生殖分化。(C)2009爱思唯尔有限公司版权所有。
In many social hymenopteran species, workers possess functional ovaries that are physiologically inactive in the presence of queens. We investigated the ovarian regulatory mechanism of workers and reproductives in a queenless ponerine ant, Diacamma sp., using histological and molecular techniques. In this ant, clear reproductive differentiation occurs via a highly sophisticated dominance behavioral interaction called "gemmae mutilation". This clear and rapid bifurcation of reproductive physiology allows us to elucidate the detailed ovarian differentiation process. Histological characteristics of functional ovaries (fusomes and ring canals) were found in both workers and reproductives, suggesting that early oogenesis is not blocked in workers. Since insulin/insulin-like growth factor signaling (IIS) is known to control insect reproduction, orthologs of 2 positive Its regulators, insulin receptor and serine-threonine kinase Akt (protein kinase B), were cloned in Diacamma (DiaInR, DiaAkt); their expression patterns during reproductive differentiation were examined by real-time quantitative polymerase chain reaction; DiaInR and DiaAkt were strongly expressed in the gasters of reproductives. Whole-mount in situ hybridization of ovaries indicated that DiaInR and DiaAkt were expressed in nurse cells, oocytes, and upper germarial regions of reproductives but not of workers. Our data suggest that the IIS pathway accounts for reproductive differentiation in late oogenesis. (C) 2009 Elsevier Ltd. All rights reserved.