High-performance liquid chromatographic application of the Hummel and Dreyer method for the determination of colchicine-tubulin binding parameters.
High-performance liquid chromatographic application of the Hummel and Dreyer method for the determination of colchicine-tubulin binding parameters.
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应用 Hummel 和 Dreyer 方法的高效液相色谱法测定秋水仙碱-微管蛋白结合参数。
DOI：
10.1016/s0021-9673(01)90886-6
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Robinson,AK
中科院分区：
文献类型：
--
作者：
Williams,RF;Aivaliotis,MJ;Barnes,LD;Robinson,AK
应用为高效液相色谱法改进的Hummel和Dreyer凝胶色谱法测定了25°C下秋水仙素-微管蛋白相互作用的解离常数。所获得的结果与其他平衡和非平衡技术的结果进行比较，并表明，秋水仙素与微管蛋白的初始相互作用必须是快速的，可能是可逆的。这种快速和灵敏的技术，它不需要放射性同位素的结合参数的测量，将是非常有用的表征微管蛋白配体相互作用。
An application of the Hummel and Dreyer gel chromatography procedure modified for high-performance liquid chromatography has been used to determine the dissociation constant for the colchicine—tubulin interaction at 25°C. The results obtained are compared with results of other equilibrium and non-equilibrium techniques and demonstrate that the initial interaction of colchicine with tubulin must be rapid and probably reversible. This rapid and sensitive technique, which does not require radioisotopes for measurement of the binding parameters, will be extremely useful for characterization of tubulin—ligand interactions.