Isolation and identification of anti-proliferative peptides from Spirulina platensis using three-step hydrolysis.
Isolation and identification of anti-proliferative peptides from Spirulina platensis using three-step hydrolysis.
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DOI：
10.1002/jsfa.7815
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发表时间：
2017-02
影响因子：
4.1
通讯作者：
Zhujun Wang;Xuewu Zhang
中科院分区：
文献类型：
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作者：
Zhujun Wang;Xuewu Zhang
背景钝顶螺旋藻是一种优良的蛋白质来源（>60%），其可以被水解成生物活性肽。结果采用胃蛋白酶、胰蛋白酶和糜蛋白酶对螺旋藻全蛋白进行了提取和水解。随后，采用凝胶过滤色谱法分离水解产物，得到四个组分（Tr 1-Tr 4）。其中，Tr 2对三种癌细胞（MCF-7、HepG-2和SGC-7901）的抗增殖活性最强，IC 50值分别为<31.25、36.42和48.25 µg mL-1。此外，一个新的肽，HVLSRAPR，鉴定从馏分Tr 1。该肽对HT-29癌细胞表现出强烈的抑制作用，IC 50值为99.88 µg mL-1。结论这些多肽具有抗肿瘤细胞增殖活性和较低的细胞毒活性，有望作为天然抗癌药物应用于保健品和医药工业。© 2016化学工业协会。
BACKGROUND Spirulina platensis is an excellent source of proteins (>60%) that can be hydrolyzed into bioactive peptides. RESULTS In this study, whole proteins of Spirulina platensis were extracted and hydrolyzed using three gastrointestinal endopeptidases (pepsin, trypsin and chymotrypsin). Subsequently, gel filtration chromatography was employed to separate hydrolysates, and four fractions (Tr1-Tr4) were obtained. Among them, Tr2 showed the strongest anti-proliferation activities on three cancer cells (MCF-7, HepG-2 and SGC-7901), with IC50 values of <31.25, 36.42 and 48.25 µg mL-1 , respectively. Furthermore, a new peptide, HVLSRAPR, was identified from fraction Tr1. This peptide exhibited strong inhibition on HT-29 cancer cells with an IC50 value of 99.88 µg mL-1 . CONCLUSION Taken together, these peptides possessed anti-proliferation activities on cancer cells and low cytotoxicity on normal cells, suggesting that they might serve as a natural anticancer agent for nutraceutical and pharmaceutical industries. © 2016 Society of Chemical Industry.