Nondestructive isolation of single cultured animal cells by femtosecond laser-induced shockwave
Nondestructive isolation of single cultured animal cells by femtosecond laser-induced shockwave
复制标题
DOI：
10.1007/s00339-004-2823-7
复制
发表时间：
2004-09-01
影响因子：
2.7
通讯作者：
Masuhara, H
中科院分区：
文献类型：
--
作者：
Hosokawa, Y;Takabayashi, H;Masuhara, H
通过在显微镜下将强飞秒激光聚焦到培养基上而产生的冲击波被用来操纵单个培养的动物细胞。小鼠 NIH3T3 成纤维细胞在胶原基质上培养，并放置在包含强飞秒激光的显微镜装置上。将激光直接聚焦在细胞上，切割丝状伪足后，细胞通过冲击波从基质上单独分离。分离后细胞的外观和运动用CCD相机监测。脱落细胞的丝状伪足再生，脱落后4小时内细胞再次粘附至基质。当以0.72μJ/脉冲的脉冲能量诱导冲击波时，80%的细胞以非破坏性方式成功地从培养板上脱离。分离细胞所需的力估计为几μN/mum(2)，这比传统激光捕获产生的光子力更大。
A shockwave induced by focusing an intense femtosecond laser onto a culture medium under a microscope was used to manipulate single cultured animal cells. Mouse NIH3T3 fibroblasts were cultured on a collagen matrix and placed on a microscope installation that included an intense femtosecond laser. Cells were detached individually from the matrix by the shockwave, after their filopodia were cut by focusing the laser directly on them. The appearance and locomotion of cells after detachment was monitored with a CCD camera. Filopodia of the detached cells were regenerated, and the cell adhered again to the matrix within 4 hrs after detachment. When the shockwave was induced at a pulse energy of 0.72 muJ/pulse, 80% of cells were successfully detached from the culture plate in a non-destructive manner. The force required to detach a cell was estimated to be a few muN/mum(2), which is larger than the photon force resulting from conventional laser trapping.