The birth of an alternatively spliced exon:: 3′ splice-site selection in Alu exons
The birth of an alternatively spliced exon:: 3′ splice-site selection in Alu exons
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DOI：
10.1126/science.1082588
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发表时间：
2003-05-23
期刊：
影响因子：
56.9
通讯作者：
Ast, G
中科院分区：
文献类型：
--
作者：
Lev-Maor, G;Sorek, R;Ast, G
AlU重复元件可以通过剪接介导的称为外切的过程插入到成熟的信使RNA中。为了了解内含子AlU转变为新外显子的分子基础和调控过程，我们编辑和分析了人类外显子AlU的数据集。我们揭示了在选择性剪接过程中控制这些外显子上3‘剪接位点选择的机制。在这些发现的基础上，我们发现了激活沉默内含子Alu外化的突变。
Alu repetitive elements can be inserted into mature messenger RNAs via a splicing-mediated process termed exonization. To understand the molecular basis and the regulation of the process of turning intronic Alus into new exons, we compiled and analyzed a data set of human exonized Alus. We revealed a mechanism that governs 3' splice-site selection in these exons during alternative splicing. On the basis of these findings, we identified mutations that activated the exonization of a silent intronic Alu.