Isotope sample preparation of diatoms for paleoenvironmental research.

Isotope sample preparation of diatoms for paleoenvironmental research.
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用于古环境研究的硅藻同位素样品制备。

DOI:
10.1371/journal.pone.0281511
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发表时间:
2023
期刊:
影响因子:
3.7
通讯作者:
--
中科院分区:
综合性期刊3区
文献类型:
--
作者:

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硅藻的同位素越来越多地用于湖泊和海洋环境的古环境研究,从而能够重建一系列变量,包括温度、降水、盐度、冰川流量、碳动力学和生物地球化学循环。本协议详细介绍了从沉积物样品中提取硅藻进行同位素分析的优化方法,使用一系列化学和密度分离技术,最大限度地减少样品损失,避免了对昂贵设备的需求。虽然设计用于提取硅藻以进行氧、硅和碳同位素分析,但概述了用于分析古群落日益感兴趣的其他同位素(例如硼和锌)的附加阶段。该方案还包括评估样品纯度的程序,以确保分析的样品产生可靠的古环境重建。总的来说,该方法旨在通过最大限度地提高样品纯度和提取过程的效率,提高硅藻同位素古环境研究的质量。
Isotopes in diatoms are increasingly used in palaeoenvironmental studies in both lacustrine and marine settings, enabling the reconstruction of a range of variables including temperature, precipitation, salinity, glacial discharge, carbon dynamics and biogeochemical cycling. This protocol details an optimised methodology for extracting diatoms for isotope analysis from sediment samples, using a range of chemical and density separation techniques that minimise sample loss and avoids the need for expensive equipment. Whilst designed for the extraction of diatoms for oxygen, silicon and carbon isotope analysis, additional stages are outlined for the analysis of other isotopes that are of increasing interest to the palaeo community (e.g., boron and zinc). The protocol also includes procedures for assessing sample purity, to ensure that analysed samples produce robust palaeoenvironmental reconstruction. Overall, the method aims to improve the quality of palaeoenvironmental research derived from isotopes in diatoms by maximising sample purity and the efficiency of the extraction process.
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