Cytokine profile in synovial fluid from patients with internal derangement of the temporomandibular joint: a preliminary study
Cytokine profile in synovial fluid from patients with internal derangement of the temporomandibular joint: a preliminary study
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DOI：
10.1259/dmfr/77288976
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发表时间：
2006-11-01
影响因子：
3.3
通讯作者：
Otsuka, K.
中科院分区：
文献类型：
--
作者：
Matsumoto, K.;Honda, K.;Otsuka, K.
目的：颞下颌关节紊乱病（Temporomandibular joint disorders，TMD）是一组颞下颌关节（Temporomandibular joint，TMJ）的慢性疼痛性疾病。虽然TMD和颞下颌关节内部紊乱之间的关联是有据可查的，但功能相关性仍不清楚。在患有TMD的受影响患者的滑液中已经确定了增加的炎症介质浓度，这表明潜在的退行性或炎症过程。本研究的目的是产生一个全面的细胞因子的表达谱在TMD.Methods：15个样本与TMJ的内部紊乱患者进行了分析，使用一种新的细胞因子阵列，使79种不同的细胞因子同时分析。在大多数滑液样品中，血管生成素（Ang）、成纤维细胞生长因子（FGF）-9、胰岛素样生长因子结合蛋白（IGFBP）-3、白细胞介素（IL）-1 α、IL-1 β、IL-8、诱导蛋白（IP）-10、巨噬细胞炎性蛋白（MIP）-1 β、骨保护素（OPG）、转化生长因子（TGF）-β 2、金属蛋白酶组织抑制剂（TIMP）-1、TIMP-2、肿瘤坏死因子（TNF）-β和血管内皮生长因子（VEGF）可检测。Ang、脑源性神经营养因子（BDNF）、FGF-4、FGF-9、IGFBP-2、IL-8、MIP-1 β、OPG、肺激活调节蛋白（PARC）、TGF-β 2、TIMP-2和VEGF的表达水平与JE的存在显著相关;其中9种细胞因子（Ang、BDNF、FGF-4、FGF-9、IGFBP-2、MIP-1 β、PARC、TGF-β 2和TIMP-2）在TMD中未被描述。此外，我们确定了先前未描述的细胞因子，这些细胞因子上调并与JE的存在显著相关。我们能够确定新的细胞因子，迄今尚未被描述在TMD。针对已鉴定的细胞因子的策略可能代表TMD的新的治疗选择。
Objectives: Temporomandibular joint disorders (TMD) comprise a group of chronic painful conditions of mastication in the temporomandibular joint (TMJ). Although the association between TMD and internal derangement of the TMJ is well documented, the functional relevance is still unclear. Increased concentrations of inflammatory mediators have been identified in the synovial fluid of affected patients with TMD, suggesting an underlying degenerative or inflammatory process. The aim of this study was to generate a comprehensive cytokine expression profile in TMD.Methods: 15 samples from patients with internal derangement of TMJ were analysed using a novel cytokine array that enables the analysis of 79 different cytokines simultaneously.Results: Cytokine levels were correlated with the presence of joint effusion (JE) determined by MRI. In the majority of synovial fluid samples, angiogenin (Ang), fibroblast growth factor (FGF)-9, insulin-like growth factor-binding protein (IGFBP)-3, interleukin (IL)-1 alpha, IL-1 beta, IL-8, inducible protein (IP)-10, macrophage inflammatory protein (MIP)-1 beta, osteoprotegerin (OPG), transforming growth factor (TGF)-beta 2, tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, tumour necrosis factor (TNF)-beta and vascular endothelial growth factor (VEGF) were detectable. Furthermore, the expression levels of Ang, brain-derived neurotrophic factor (BDNF), FGF-4, FGF-9, IGFBP-2, IL-8, MIP-1 beta, OPG, pulmonary and activation-regulated protein (PARC), TGF-beta 2, TIMP-2 and VEGF were significantly associated with the presence of JE; among these, nine cytokines (Ang, BDNF, FGF-4, FGF-9, IGFBP-2, MIP-1 beta, PARC, TGF-beta 2 and TIMP-2) were hitherto not described in TMD.Conclusions: This study confirmed previous reports of elevated cytokine levels in TMD. Additionally, we identified previously undescribed cytokines that were upregulated and correlated significantly with the presence of JE. We were able to identify novel cytokines that have hitherto not been described in TMD. Strategies targeting the identified cytokines may represent a novel therapy option in TMD.