A gastrin gene is expressed in both porcine pituitary and antral mucosal tissues.
A gastrin gene is expressed in both porcine pituitary and antral mucosal tissues.
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胃泌素基因在猪垂体和胃窦粘膜组织中表达。
DOI：
10.1093/nar/13.20.7299
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发表时间：
1985
影响因子：
14.9
通讯作者：
Agarwal,K
中科院分区：
文献类型：
--
作者：
Powell,CT;Ney,C;Aran,P;Agarwal,K
通过对猪大脑皮质、小脑、下丘脑、垂体、肾脏、肝脏、胰腺、肠道和胃窦粘膜的总RNA进行S1核酸酶作图，研究了胃泌素基因在这些组织中的表达。我们的结果表明，胃泌素基因只在胃窦粘膜和垂体组织中表达。根据在给定重量的总RNA存在下防止S1核酸酶消化的胃泌素特异性探针的量，垂体中存在的胃泌素mRNA的量大约是胃窦粘膜的330倍。这些发现有助于建立胃泌素基因表达的组织分布。
By employing S1 nuclease mapping of total RNA isolated from porcine cerebral cortex, cerebellum, hypothalamus, pituitary, kidney, liver, pancreas, intestine, and antral mucosa, we have investigated gastrin gene expression in these tissues. Our results show that a gastrin gene is expressed only in the antral mucosal and pituitary tissues. Based on the amount of gastrin specific probe protected from S1 nuclease digestion in the presence of a given weight of total RNA, the amount of gastrin mRNA present in pituitary is approximately 330 times lower than in antral mucosa. These findings help establish the tissue distribution of gastrin gene expression.