Rax1, a protein required for the establishment of the bipolar budding pattern in yeast
Rax1, a protein required for the establishment of the bipolar budding pattern in yeast
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DOI：
10.1016/j.gene.2003.11.021
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发表时间：
2004-03-03
期刊：
影响因子：
3.5
通讯作者：
Chant, J
中科院分区：
文献类型：
--
作者：
Fujita, A;Lord, M;Chant, J
在酿酒酵母中，细胞类型决定了两种不同的空间出芽模式。单倍体细胞表现出轴向模式，而二倍体细胞表现出双极模式。Ax 11是胰岛素降解酶（IDE）家族的成员，是单倍体轴型的关键形态决定因子。在这里，我们确定了一个新的基因，RAX 1，特别是需要双极出芽模式。RAX 1的缺失改变了ax 11单倍体的双极模式，导致轴向模式的逆转，也改变了bud 3和bud 4单倍体的双极模式。而bud 10 rax 1单倍体则表现为随机出芽模式，表明bud 10是轴向出芽的关键近端标志。需要Rax 1来定位Bud 8（远端双极出芽标志）。有趣的是，Rax 1含有一个C-末端结构域，与胰岛素相关肽具有一定的相似性。我们的研究结果表明，Rax 1是必要的双极萌芽标志的建立。(C)2004 Elsevier B. V.保留所有权利。
In Saccharomyces cerevisiae, cell type determines two distinct spatial budding patterns. Haploid cells exhibit an axial pattern, whereas diploid cells exhibit a bipolar pattern. Ax11, a member of the insulin-degrading enzyme (IDE) family, is the key morphological determinant for the haploid axial pattern. Here we identified a novel gene, RAX1, specifically required for the bipolar budding pattern. Loss of RAX1 alters the bipolar pattern of ax11 haploids resulting in reversion to the axial pattern, and also alters the bipolar patterns of bud3 and bud4 haploids. However, bud10 rax1 haploids exhibiva random budding pattern, suggesting Bud10 acts as the key proximal landmark in axial budding. Rax1 is required for the localization of Bud8, the distal bipolar budding landmark. Interestingly, Rax1 contains a C-terminal domain possessing some similarity to insulin-related peptides. Our results suggest that Rax1 is necessary for the establishment of the bipolar budding landmark. (C) 2004 Elsevier B.V. All rights reserved.