Identification of the major mannose-binding proteins from chicken egg yolk and chicken serum as immunoglobulins.
Identification of the major mannose-binding proteins from chicken egg yolk and chicken serum as immunoglobulins.
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将鸡蛋黄和鸡血清中的主要甘露糖结合蛋白鉴定为免疫球蛋白。
DOI：
10.1073/pnas.83.24.9670
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发表时间：
1986
影响因子：
11.1
通讯作者：
Lee,YC
中科院分区：
文献类型：
--
作者：
Wang,KY;Hoppe,CA;Datta,PK;Fogelstrom,A;Lee,YC
鸡蛋黄中含有一种甘露糖结合蛋白，可以通过对鸡血清甘露糖结合蛋白的亲和层析和凝胶过滤方法进行改进来纯化。蛋黄蛋白与血清蛋白在表观分子量（169 +/- 7 kDa）、巯基乙醇变性后产生的亚基（74 kDa和27 kDa，比例约为1：1）、与鸡血清甘露糖结合蛋白抗体的免疫反应性、氨基酸组成、结合的最佳pH值、钙独立性、糖结合特异性和特异性结合活性方面无法区分。此外，鸡甘露糖结合蛋白与抗鸡IgG的γ链特异性抗体发生交叉反应。通过其他几项标准将结合蛋白鉴定为IgG：还原和非还原NaDodSO 4/聚丙烯酰胺凝胶电泳时的电泳图谱相同，Fractogel TSK HW-55（F）柱上的共色谱，相似的氨基酸组成，以及从纯化血清IgG中分离甘露糖结合蛋白的产率与全血清相当。这些结果支持这样的观点，即来自鸡血清和蛋黄的甘露糖结合蛋白是相似的，如果不是相同的，并且是鸡IgG的子集。
Chicken egg yolk contains a mannose-binding protein that could be purified with a modification of the procedure of affinity chromatography and gel filtration used for chicken serum mannose-binding protein. The yolk protein was indistinguishable from the serum protein with respect to apparent molecular masses (169 +/- 7 kDa), subunits (74 kDa and 27 kDa, in approximately 1:1 ratio) produced after denaturation in the presence of mercaptoethanol, immunoreactivity with antibody against the chicken serum mannose-binding protein, amino acid composition, pH optimum for binding, calcium independence of binding, sugar-binding specificity, and specific-binding activity. Moreover, the chicken mannose-binding proteins cross-reacted with gamma-chain-specific antibody against chicken IgG. The binding proteins were identified as IgGs by several other criteria: identical electrophoresis pattern when subjected to reducing and nonreducing NaDodSO4/polyacrylamide gel electrophoresis, cochromatography on a Fractogel TSK HW-55(F) column, similar amino acid composition, and isolation of mannose-binding protein from purified serum IgG at a yield comparable to whole serum. These results support the notion that the mannose-binding proteins from the chicken serum and egg yolk are similar, if not identical, and are a subset of chicken IgG.