Prevalence and relevance of EBV latency in nasopharyngeal carcinoma in Israel
Prevalence and relevance of EBV latency in nasopharyngeal carcinoma in Israel
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DOI：
10.1136/jcp.2003.013094
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发表时间：
2004-03-01
影响因子：
3.4
通讯作者：
Ben-Izhak, O
中科院分区：
文献类型：
--
作者：
Bar-Sela, G;Kuten, A;Ben-Izhak, O
背景：鼻咽癌(NPC)常与EB病毒(EBV)相关。鼻咽癌在西方国家的发病率低于远东地区，EBV潜伏期在鼻咽癌中的发病率较低。以色列作为地中海地区的一部分，是鼻咽癌的中等风险国家之一。方法：采用潜伏膜蛋白1(LMP-1)免疫组织化学(IHC)和EBV编码RNA(EBER)原位杂交法(ISH)评价EBV潜伏期在以色列鼻咽癌患者中的发生率和可能的预后价值。结果：LMP-1免疫组化仅在6例鼻咽癌标本中呈阳性表达，均为未分化的组织学类型。在45份样本中，有40份EBERISH呈阳性。根据组织学类型，5例鳞癌中3例EBV阳性，40例非角化性未分化癌中37例EBV阳性。虽然EBV在非鳞癌患者中更为常见，但差异并不显著，可能是因为角化癌患者的数量较少。EBER ISH阳性和阴性患者在临床类型和生存期方面无显著差异。未发现EBV潜伏期与患者性别、年龄、来源、分期或生存期之间的关系。结论：EBV潜伏期与以色列鼻咽癌的EBV潜伏期高度相关。在同一组患者中，LMP-1免疫组化法检测鼻咽癌中EBV潜伏期的敏感性显著降低。
Background: Nasopharyngeal carcinoma (NPC) is frequently associated with Epstein-Barr virus (EBV). The incidence of NPC in Western countries is lower than in the Far East, and EBV latency in NPC is less prevalent. Israel, as a part of the Mediterranean area, is one of the countries with an intermediate risk for NPC.Methods: Immunohistochemistry (IHC) for latent membrane protein 1 (LMP-1) and in situ hybridisation (ISH) for EBV encoded RNA ( EBER) were used to evaluate the prevalence and possible prognostic value of EBV latency among Israeli patients with NPC. Forty five patients with different NPC histologies were studied.Results: LMP-1 IHC was positive in six samples only, all with undifferentiated histology. EBER ISH was positive in 40 of the 45 samples. According to histological type, three of five patients with squamous cell carcinoma were EBV positive and 37 of 40 non-keratinising and undifferentiated carcinoma cases were positive. Although EBV was more prevalent in patients with non-squamous carcinoma, the difference was not significant, probably because of the small number of patients with keratinising carcinoma. With regard to the clinical categories and survival, no significant difference could be detected between patients who were positive or negative for EBER ISH. No association was found between EBV latency and patient sex, age, origin, stage, or survival.Conclusions: NPC in Israel is highly associated with EBV latency as detected by EBER ISH. LMP-1 IHC is considerably less sensitive in detecting EBV latency in NPC among the same patient group.