Application of waste frying oils in the biosynthesis of biodemulsifier by a demulsifying strain Alcaligenes sp. S-XJ-1.
Application of waste frying oils in the biosynthesis of biodemulsifier by a demulsifying strain Alcaligenes sp. S-XJ-1.
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DOI：
10.1016/s1001-0742(10)60508-6
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发表时间：
2011-06
影响因子：
6.9
通讯作者：
Jia Liu;Kaiming Peng;Xiang-feng Huang;Li-jun Lu;Hang Cheng;Dianhai Yang;Qi Zhou;Huiping Deng
中科院分区：
文献类型：
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作者：
Jia Liu;Kaiming Peng;Xiang-feng Huang;Li-jun Lu;Hang Cheng;Dianhai Yang;Qi Zhou;Huiping Deng
生物破乳剂的探索已成为一个新的研究方向。以废弃煎炸油（WFO）为碳源合成生物破乳剂具有降低生产成本、提高生物破乳剂在油田应用的潜在前景。在这项研究中，破乳菌株Alcaligenessp。研究了S-XJ-1以废弃煎炸油为碳源合成生物破乳剂。研究发现，与以石蜡为碳源相比，提高培养基初始pH可以提高生物破乳剂的产率，但破乳率降低。此外，以废煎炸油和石蜡为混合碳源生产的生物破乳剂与以石蜡或废煎炸油作为单一碳源生产的生物破乳剂相比，其破乳能力较低。使用废煎炸油作为补充碳源的生物破乳剂分批补料发酵被发现是一种合适的方法。以三棕榈酸甘油酯、油精和三硬脂酸甘油酯为唯一碳源合成生物破乳剂，研究了废弃煎炸油的利用机理。结果表明，饱和长链脂肪酸是S-XJ-1难以利用的成分，但能有效增强所制备的生物破乳剂的破乳能力。此外，FT-IR结果表明，生物破乳剂的破乳能力与C=O基团和氮元素的含量有关。
Exploration of biodemulsifiers has become a new research aspect. Using waste frying oils (WFOs) as carbon source to synthesize biodemulsifiers has a potential prospect to decrease production cost and to improve the application of biodemulsifiers in the oilfield. In this study, a demulsifying strain,Alcaligenessp. S-XJ-1, was investigated to synthesize a biodemulsifier using waste frying oils as carbon source. It was found that the increase of initial pH of culture medium could increase the biodemulsifier yield but decrease the demulsification ratio compared to that using paraffin as carbon source. In addition, a biodemulsifier produced by waste frying oils and paraffin as mixed carbon source had a lower demulsification capability compared with that produced by paraffin or waste frying oil as sole carbon source. Fed-batch fermentation of biodemulsifier using waste frying oils as supplementary carbon source was found to be a suitable method. Mechanism of waste frying oils utilization was studied by using tripalmitin, olein and tristearin as sole carbon sources to synthesize biodemulsifier. The results showed saturated long-chain fatty acid was difficult for S-XJ-1 to utilize but could effectively enhance the demulsification ability of the produced biodemulsifier. Moreover, FT-IR result showed that the demulsification capability of biodemulsifiers was associated with the content of C=O group and nitrogen element.