In vivo assessment of skin electroporation using square wave pulses
In vivo assessment of skin electroporation using square wave pulses
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DOI：
10.1016/s0168-3659(01)00548-x
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发表时间：
2002-02-19
影响因子：
10.8
通讯作者：
Préat, V
中科院分区：
文献类型：
--
作者：
Dujardin, N;Staes, E;Préat, V
经证明，对皮肤施加短时高强度脉冲可将透皮药物输送增强几个数量级，并使组织中的细胞瞬时通透。指数衰减 (ED) 脉冲和方波 (SW) 脉冲均已应用。后者也已用于电化学疗法。迄今为止。尚未分析它们对皮肤完整性的影响。这项工作的范围是 (l) 研究 SW 脉冲对角质层和皮肤的影响，(ii) 评估与电穿孔相关的安全问题，(iii) 有助于理解药物转运。生物物理技术（经皮失水、色度测定、阻抗和激光多普勒测速或成像测量）和组织学方法相结合，提供了效果的全局图景。施加到皮肤上的十次短波脉冲会导致皮肤屏障功能轻度受损，皮肤电阻急剧下降。这些变化是可逆的。短暂减少（
The application of short-duration high-vintage pulses to the skin has been shown to enhance transdermal drug delivery by several orders of magnitude and to transiently permeabilize cells in tissue. Both exponentially decaying (ED) pulses and square wave (SW) pulses have been applied. The latter have also been used for electrochemotherapy. To date. their effect on skin integrity has not been analyzed. The scope of this work was (l) to investigate the effect induced by SW pulses on the stratum corneum and the skin, (ii) to evaluate the safety issue associated with electroporation, (iii) to contribute to the understanding of drug transport. Biophysical techniques (transepidermal water loss, chromametry, impedance and laser Doppler velocimetry or imaging measurement) and histological methods were combined to provide a global picture of the effects. Ten SW pulses applied to the skin induced a mild impairment of the skin barrier function and a dramatic decrease in skin resistance. These changes were reversible. A transient decrease (