Cloning and homology modeling of a serine protease gene (PrC) from the nematophagous fungus Clonostachys rosea
Cloning and homology modeling of a serine protease gene (PrC) from the nematophagous fungus Clonostachys rosea
复制标题
食线虫真菌 Clonostachysrosea 丝氨酸蛋白酶基因 (PrC) 的克隆和同源建模
DOI：
10.1007/s13213-010-0166-5
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发表时间：
2011-09-01
影响因子：
3
通讯作者：
Zhang, Ke-Qin
中科院分区：
文献类型：
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作者：
Liang, Lianming;Yang, Jinkui;Zhang, Ke-Qin
食草真菌Clonostachys rosea可以寄生线虫以及昆虫和几种真菌。蛋白酶在感染过程中起着关键作用，被认为是这些真菌中重要的毒力因子。本研究首次从C.玫瑰色。该基因含有3个内含子和4个外显子，编码386个氨基酸残基的多肽。成熟的蛋白质是277个氨基酸残基长，并包含一个保守的基序共享的肽酶S8家族成员。其N-末端氨基酸残基与食虫真菌和昆虫病原真菌的丝氨酸蛋白酶具有高度的序列相似性。根据PrC蛋白的氨基酸序列，预测了其三维结构，并与蛋白酶K的三维结构进行了比较。我们的研究结果为进一步了解C.玫瑰花线虫感染。这些知识可以通过基因工程来提高利用真菌感染控制寄生线虫的有效性。
The nematophagous fungus Clonostachys rosea can parasitize nematodes as well as insects and several fungi. Proteases play critical roles during infection and are considered important virulence factors in these fungi. In this study, a cuticle-degrading serine protease gene (PrC) was cloned for the first time from C. rosea. The gene contained three introns and four exons and encodes a polypeptide of 386 amino acid residues. The mature protein is 277 amino acid residues long and contains a conserved motif shared by peptidase S8 family members. Its N-terminal amino acid residues showed a high degree of sequence similarity with serine proteases from nematophagous and entomopathogenic fungi. Based on the PrC amino acid sequence, the three-dimensional structure has been predicted and compared with that of protease K. Our results provide a basis for further understanding the molecular mechanism of C. rosea infection of nematodes. Such knowledge could be explored for improving by genetic engineering the effectiveness of the use of fungal infections to control parasitic nematodes.