Severity of the streptomycin resistance and streptomycin dependence phenotypes of ribosomal protein S12 of Thermus thermophilus depends on the identity of highly conserved amino acid residues
Severity of the streptomycin resistance and streptomycin dependence phenotypes of ribosomal protein S12 of Thermus thermophilus depends on the identity of highly conserved amino acid residues
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DOI：
10.1128/jb.187.10.3548-3550.2005
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发表时间：
2005-05-01
影响因子：
3.2
通讯作者：
Dahlberg, AE
中科院分区：
文献类型：
--
作者：
Carr, JF;Gregory, ST;Dahlberg, AE
核糖体蛋白S12突变体链霉素依赖表型的结构基础尚不清楚。在这里，我们描述了嗜热热菌rpsL的定点诱变和基因替换的应用，以评估侧链身份和三级相互作用作为药物依赖性突变表型决定因素的重要性。
The structural basis for the streptomycin dependence phenotype of ribosomal protein S12 mutants is poorly understood. Here we describe the application of site-directed mutagenesis and gene replacement of Thermus thermophilus rpsL to assess the importance of side chain identity and tertiary interactions as phenotypic determinants of drug-dependent mutants.