Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3
Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3
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DOI：
10.1016/j.febslet.2012.08.012
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发表时间：
2012-09-21
期刊：
影响因子：
3.5
通讯作者：
Archangelo, Leticia Froehlich
中科院分区：
文献类型：
--
作者：
Bigarella, Carolina L.;Vieira Ferro, Karla P.;Archangelo, Leticia Froehlich
ARHGAP21是一个217 kDa的RhoGAP蛋白，通过控制Cdc42和FAK活性来调节细胞迁移。本研究用质谱法鉴定了250 kDa-ARHGAP21。这种修饰形式在细胞系和人原代细胞之间表达差异。共免疫沉淀和体外SUMOylation证实了SUMO2/3对ARHGAP21的特异性修饰，并将SUMOylation位点定位为ARHGAP21赖氨酸K1443。免疫荧光染色显示ARHGAP21与SUMO2/3共定位于细胞质和膜室。有趣的是，我们的研究结果表明，ARHGAP21 SUMOylation可能与细胞增殖有关。因此，ARHGAP21的SUMOylation可能是指导其功能的一种途径。(C) 2012年欧洲生化学会联合会。Elsevier B.V.版权所有。
ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa-ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co-localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.