Starvation-Survival Patterns of Sixteen Freshly Isolated Open-Ocean Bacteria
Starvation-Survival Patterns of Sixteen Freshly Isolated Open-Ocean Bacteria
复制标题
十六种新鲜分离的远洋细菌的饥饿生存模式
DOI：
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发表时间：
1983
影响因子：
4.4
通讯作者：
R. Y. Morita
中科院分区：
文献类型：
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作者：
P. Amy;R. Y. Morita
从NORPAX邮轮上分离的16株海洋分离菌，在初始分离后转移到培养基上，在营养剥夺中存活了至少8个月（最长时间试验）。除了一株外，其余的细胞都保持完整，尽管它们的大小和形状发生了很大变化，而且都变小了，大小从40%减少到79%。实验证明了三种饥饿生存模式，即(i)活细胞最初增加，然后减少，直到达到一定数量；（ii）活细胞增加，直到达到一定数量；（iii）活细胞减少，直到达到一定数量。从每种饥饿生存模式中分离出一株，饥饿8个月，然后与饥饿4个月的Ant-300进行[14C]谷氨酸摄取、呼吸和结合的比较。每种情况下对谷氨酸的反应都是快速和线性的。这些数据表明，Ant-300的饥饿生存并不是一种异常情况，开放海洋细菌可以承受长时间的营养剥夺，并且在营养可用的情况下仍然保持积极代谢的能力。
Sixteen marine isolates from a NORPAX cruise, which were transferred once on medium after initial isolation, survived nutrient deprivation for at least 8 months (longest period test). All but one isolate remained cellularly intact, although their sizes and shapes changed greatly, and all became smaller, decreasing in size from 40 to 79%. Three starvation-survival patterns were demonstrated, namely (i) an initial increase in viable cells followed by a decrease until a constant number was reached, (ii) an increase in viable cells until a constant number was reached, and (iii) a decrease in viable cells until a constant number was reached. One isolate from each starvation-survival pattern was starved for 8 months and then was tested in comparison with 4-month-starved Ant-300 for [14C]glutamic acid uptake, respiration, and incorporation. The response to glutamic acid was rapid and linear in each case. The data indicate that the starvation-survival of Ant-300 is not an anomalous situation and that open ocean bacteria can withstand nutrient deprivation for long periods of time and still retain the capacity for active metabolism, if the nutrients become available.