Preprodynorphin-Expressing Neurons Constitute a Large Subgroup of Somatostatin-Expressing GABAergic Interneurons in the Mouse Neocortex
Preprodynorphin-Expressing Neurons Constitute a Large Subgroup of Somatostatin-Expressing GABAergic Interneurons in the Mouse Neocortex
复制标题
表达前强啡肽的神经元构成小鼠新皮质中表达生长抑素的 GABA 能中间神经元的一大亚群
DOI：
10.1002/cne.23477
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
and Takeshi Kaneko
中科院分区：
文献类型：
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作者：
Jaerin Sohn;Hiroyuki Hioki;Shinichiro Okamoto;and Takeshi Kaneko
Dynorphins， leumorphin和neoendorphins是preproprodynorphin （PPD）衍生的肽和κ -阿片受体的配体。利用PPD C末端抗体，我们研究了小鼠新皮层中表达PPD的神经元的化学和分子特征。PPD‐免疫阳性神经元体最常分布在第5层，第2-4层和第6层较少分布在整个新皮质区域。免疫荧光和荧光mRNA信号的联合标记显示，几乎所有PPD免疫阳性的神经元都表达谷氨酸脱羧酶，而不表达谷氨酸囊泡转运蛋白，这表明它们具有γ -氨基丁酸（GABA）能特性，并且PPD免疫阳性的神经元占初级体感觉区GABA能中间神经元的15%。由于gaba能中间神经元被特定的标记物分成几组，我们进一步用双免疫荧光标记法检测了表达PPD‐神经元的化学特征。超过95%的PPD免疫阳性神经元在初级体感觉区、初级运动区、眼窝额叶区和初级视觉区也呈生长抑素（SOM）免疫阳性，但在内侧前额叶皮层只有24%呈SOM免疫阳性。在初级体感觉区，PPD免疫阳性神经元分别占2-3层、4层、5层和6层SOM免疫阳性神经元的50%、79%、55%和17%。虽然SOM‐表达神经元包含calretinin‐、神经肽Y‐、一氧化氮合酶‐和reelin‐表达神经元作为亚组，但在许多PPD‐免疫阳性神经元中仅检测到reelin免疫反应性。这些结果表明，表达PPD的神经元构成了表达SOM‐的皮质中间神经元的一个大亚群，表达PPD/SOM‐的gabaergy神经元可能不仅是局部皮质回路的抑制元件，而且是表达κ‐阿片样物质和/或SOM受体的皮质神经元的调节剂。中华神经科杂志，2014,22(2):1506 - 1526。©2013 Wiley期刊公司
Dynorphins, leumorphin, and neoendorphins are preprodynorphin (PPD)‐derived peptides and ligands for κ‐opioid receptors. Using an antibody to PPD C‐terminal, we investigated the chemical and molecular characteristics of PPD‐expressing neurons in mouse neocortex. PPD‐immunopositive neuronal somata were distributed most frequently in layer 5 and less frequently in layers 2–4 and 6 throughout neocortical regions. Combined labeling of immunofluorescence and fluorescent mRNA signals revealed that almost all PPD‐immunopositive neurons expressed glutamic acid decarboxylase but not vesicular glutamate transporter, indicating their γ‐aminobutyric acid (GABA)ergic characteristics, and that PPD‐immunopositive neurons accounted for 15% of GABAergic interneurons in the primary somatosensory area. As GABAergic interneurons were divided into several groups by specific markers, we further examined the chemical characteristics of PPD‐expressing neurons by the double immunofluorescence labeling method. More than 95% of PPD‐immunopositive neurons were also somatostatin (SOM)‐immunopositive in the primary somatosensory, primary motor, orbitofrontal, and primary visual areas, but only 24% were SOM‐immunopositive in the medial prefrontal cortex. In the primary somatosensory area, PPD‐immunopositive neurons constituted 50%, 79%, 55%, and 17% of SOM‐immunopositive neurons in layers 2–3, 4, 5, and 6, respectively. Although SOM‐expressing neurons contained calretinin‐, neuropeptide Y‐, nitric oxide synthase‐, and reelin‐expressing neurons as subgroups, only reelin immunoreactivity was detected in many PPD‐immunopositive neurons. These results indicate that PPD‐expressing neurons constitute a large subgroup of SOM‐expressing cortical interneurons, and the PPD/SOM‐expressing GABAergic neurons might serve not only as inhibitory elements in the local cortical circuit, but also as modulators for cortical neurons expressing κ‐opioid and/or SOM receptors. J. Comp. Neurol. 522:1506–1526, 2014. © 2013 Wiley Periodicals, Inc.