SHORT-TERM EXPOSURE TO ETHANOL CAUSES A DIFFERENTIAL RESPONSE BETWEEN NERVE GROWTH FACTOR AND BRAIN-DERIVED NEUROTROPHIC FACTOR LIGAND/RECEPTOR SYSTEMS IN THE MOUSE CEREBELLUM
SHORT-TERM EXPOSURE TO ETHANOL CAUSES A DIFFERENTIAL RESPONSE BETWEEN NERVE GROWTH FACTOR AND BRAIN-DERIVED NEUROTROPHIC FACTOR LIGAND/RECEPTOR SYSTEMS IN THE MOUSE CEREBELLUM
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DOI：
10.1016/j.neuroscience.2009.10.045
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发表时间：
2010-01-20
期刊：
影响因子：
3.3
通讯作者：
Takeuchi, Y.
中科院分区：
文献类型：
--
作者：
Wang, Z. Y.;Miki, T.;Takeuchi, Y.
酒精摄入会影响神经心理和运动功能。我们假设参与这些功能的关键因素之一是神经营养因子及其受体。因此，我们研究了短期乙醇暴露的影响，在小脑神经营养因子配体和受体的mRNA表达和蛋白质水平，使用实时RT-PCR和蛋白质印迹技术。雄性BALB/C小鼠喂食含5%（v/v）乙醇的液体饲料。配对喂养的对照小鼠喂食相同的流质饮食，不同之处在于用等热量的蔗糖代替乙醇。在本研究中使用了表现出I期或2期中毒体征的小鼠的小脑。我们发现，暴露于乙醇导致神经生长因子（NGF）和TrkA mRNA表达水平升高，但脑源性神经营养因子（BDNF）mRNA表达水平降低。TrkB和p73 mRNA的表达无变化。发现这些蛋白质水平的变化反映了这些mRNA表达水平。我们的结论是，暴露于乙醇短时间内可以引起不同的神经营养因子配体/受体系统的反应。这些变化的功能后果目前尚不清楚。(C)2010年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Alcohol ingestion affects both neuropsychological and motor functions. We hypothesized that one of the key factors involved in such functions are neurotrophins and their receptors. We have therefore examined the effects of short-term ethanol exposure on the mRNA expression and protein levels of neurotrophin ligands and receptors in the cerebellum using real-time RT-PCR and Western blotting techniques. Male BALB/C mice were fed a liquid diet containing 5% (v/v) ethanol. The pair-fed control mice were fed an identical liquid diet except that sucrose was substituted isocalorically for ethanol. The cerebellum of mice exhibiting intoxication signs of stage I or 2 were used in the present study. We found that exposure to ethanol resulted in elevated levels of nerve growth factor (NGF) and TrkA mRNA expression but a decreased level of brain-derived neurotrophic factor (BDNF) mRNA expression. The expression of TrkB and p73 mRNA was unchanged. Changes in the level of these proteins were found to mirror these mRNA expression levels. We conclude that exposure to ethanol for a short period can cause a differential responsive in the various neurotrophin ligand/receptor systems. The functional consequences of these changes are unknown at present. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.