Apoptosis regulation differs between ulcerative colitis-associated and sporadic colonic tumors. Association with survivin and bcl-2.
Apoptosis regulation differs between ulcerative colitis-associated and sporadic colonic tumors. Association with survivin and bcl-2.
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溃疡性结肠炎相关肿瘤和散发性结肠肿瘤之间的细胞凋亡调节不同。
DOI：
10.1309/ylx4-l4h3-6k54-x92h
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发表时间：
2003
影响因子：
3.5
通讯作者：
I. Okayasu
中科院分区：
文献类型：
--
作者：
T. Mikami;Tsutomu Yoshida;Fumiyuki Akino;T. Motoori;M. Yajima;I. Okayasu
为了阐明溃疡性结肠炎 (UC) 相关病变的动力学，研究了细胞增殖、凋亡和凋亡抑制蛋白的表达。对 22 例低度不典型增生 (LGD)、25 例高度不典型增生 (HGD) 和 13 例 UC 相关腺癌中的 Ki-67 标记以及生存素和 bcl-2 表达进行免疫组织化学检查，并与 21 例 LGD 散发性腺瘤、22 例 HGD 散发性腺瘤和 21 例浸润性腺癌进行比较。通过切口末端标记和单链 DNA 的免疫组织化学分析来研究细胞凋亡。在UC相关的LGD中，Ki-67阳性细胞在隐窝的下半部比上半部更常见，这与bcl-2表达有关，而在散发性腺瘤中，此类细胞在上半部更常见。 UC 相关 LGD 与 LGD 散发性腺瘤之间或 UC 相关 HGD 与 HGD 散发性腺瘤之间未发现细胞凋亡差异。然而，UC 相关癌的细胞凋亡计数低于散发性侵袭性癌。这似乎与较高的生存素表达有关，而两种类型的侵袭性病变之间的 bcl-2 水平没有显着差异。与散发性浸润性结肠癌相比，UC 相关的细胞凋亡频率较低，这与生存素表达升高有关。两种类型的肿瘤发生过程中细胞凋亡的控制可能不同。
To clarify kinetics in ulcerative colitis (UC)-associated lesions, cell proliferation, apoptosis, and expression of apoptosis-inhibitory proteins were studied. Ki-67 labeling and survivin and bcl-2 expression were examined immunohistochemically in 22 low-grade dysplasias (LGDs), 25 high-grade dysplasias (HGDs), and 13 adenocarcinomas associated with UC, and for comparison in 21 sporadic adenomas with LGD, 22 sporadic adenomas with HGD, and 21 invasive adenocarcinomas. Apoptosis was studied with nick-end labeling and immunohistochemical analysis of single-stranded DNA. In UC-associated LGDs, Ki-67--positive cells were more frequent in the lower than the upper half of the crypt, related to bcl-2 expression, while in sporadic adenomas such cells were more common in the upper half. No difference in apoptosis was found between UC-associated LGDs and sporadic adenomas with LGD or between UC-associated HGDs and sporadic adenomas with HGD. However, UC-associated carcinomas exhibited a lower apoptotic count than their sporadic invasive counterparts. This seemed related to higher survivin expression without a significant difference between the 2 types of invasive lesions regarding bcl-2 levels. Apoptosis is less frequent in UC-associated than in sporadic invasive colon carcinomas, this being linked to elevated survivin expression. The control of apoptosis may be different in the 2 types of tumorigenesis.
影响因子：
11.2
作者：
L. Tarmin;Jing Yin;N. Harpaz;M. Kozam;Jeroen Noordzij;Lilian B. Antonio;H. Jiang;O. Chan;K. Cymes;S. Meltzer
通讯作者：
L. Tarmin;Jing Yin;N. Harpaz;M. Kozam;Jeroen Noordzij;Lilian B. Antonio;H. Jiang;O. Chan;K. Cymes;S. Meltzer
DOI：
--
发表时间：
1999-11
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Dario C. Altieri;Pier Carlo Marchisio
通讯作者：
Dario C. Altieri;Pier Carlo Marchisio