Soap chromatography—a new high-performance liquid chromatographic technique for separation of ionizable materials
Soap chromatography—a new high-performance liquid chromatographic technique for separation of ionizable materials
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肥皂色谱——一种用于分离可电离物质的新型高效液相色谱技术
DOI：
10.1016/s0021-9673(00)82234-7
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发表时间：
1976
影响因子：
4.1
通讯作者：
G. R. Laird
中科院分区：
文献类型：
--
作者：
J. Knox;G. R. Laird
描述了一种新型的高性能离子对色谱法，其中在丙醇-水洗脱液中添加约 1% 的洗涤剂（十六烷基三甲基溴化铵）。柱填料可以是反相材料（例如 SAS 硅胶）或硅胶（例如 Partisil）。该方法可以对多种磺酸和含有 1 至 3 个 -SO3H 基团的衍生染料进行高分辨率分离。保留程度可以通过改变西曲溴铵浓度、洗脱液的酸度、浓度和添加剂的性质来改变。在反相系统中，西曲溴铵-磺酸根离子对很可能从富含水的洗脱液中萃取到富含丙醇和西曲溴铵的吸附层中。在硅胶系统中，离子对可能存在于洗脱相中，并被吸附到硅胶表面上，丙醇充当离子对和表面的保护剂。塔板高度方面的色谱柱性能与普通吸附色谱中获得的性能相当。显示了萘胺磺酸异构体（包括 J-酸和 γ-酸）以及日落黄与杂质的具体分离。
A new form of high-performance ion-pair chromatography is described in which a detergent (cetyltrimethylammonium bromide) is added at around the 1% level to a propanol-water eluent. The column packing may either be a reversed-phase material (e.g., SAS silica) or a silica gel (e.g., Partisil). The method allows high-resolution separations of a wide range of sulphonic acids and derived dyestuffs containing one to three −SO3H groups. The degree of retention can be varied by changing cetrimide concentration, acidity of eluent, concentrations, and nature of additives.In the reversed-phase system it is probable that cetrimide-sulphonate ion pairs are extracted from the water-rich eluent into an adsorbed layer rich in propanol and cetrimide. In the silica gel system ion pairs are probably present in the eluent phase and are adsorbed onto the silica gel surface with propanol acting as a protective agent for both ion pairs and surface. Column performance in terms of plate height is equivalent to that obtainable in normal adsorption chromatography. Specific separations are shown of naphthylaminesulphonic acid isomers, including J-acid and γ-acid, and of sunset yellow from impurities.