Enzymatic detachment of Staphylococcus epidermidis biofilms
Enzymatic detachment of Staphylococcus epidermidis biofilms
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DOI：
10.1128/aac.48.7.2633-2636.2004
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发表时间：
2004-07-01
影响因子：
4.9
通讯作者：
Ramasubbu, N
中科院分区：
文献类型：
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作者：
Kaplan, JB;Ragunath, C;Ramasubbu, N
革兰氏阳性细菌表皮葡萄球菌是与导管和其他留置医疗器械相关的感染的最常见原因。S.表皮细胞产生细胞外粘液，使其能够在塑料表面上形成粘附的生物膜。我们发现，由革兰氏阴性牙周病原菌伴放线放线杆菌产生的生物膜释放酶可以快速有效地清除S。塑料表面的表皮生物膜。这种酶通过释放S.表皮细胞用酶预涂表面可以防止S。表皮生物膜形成。我们的研究结果表明，生物膜释放酶可以表现出广谱活性，这些酶可能是有用的，作为生物膜剂。
The gram-positive bacterium Staphylococcus epidermidis is the most common cause of infections associated with catheters and other indwelling medical devices. S. epidermidis produces an extracellular slime that enables it to form adherent biofilms on plastic surfaces. We found that a biofilm-releasing enzyme produced by the gram-negative periodontal pathogen Actinobacillus actinomycetemcomitans rapidly and efficiently removed S. epidermidis biofilms from plastic surfaces. The enzyme worked by releasing extracellular slime from S. epidermidis cells. Precoating surfaces with the enzyme prevented S. epidermidis biofilm formation. Our findings demonstrate that biofilm-releasing enzymes can exhibit broad-spectrum activity and that these enzymes may be useful as antibiofilm agents.