Hexachlorocyclohexanes, potent stimuli of O2- production and calcium release in human polymorphonuclear leukocytes.
Hexachlorocyclohexanes, potent stimuli of O2- production and calcium release in human polymorphonuclear leukocytes.
复制标题
六氯环己烷，有效刺激人类多形核白细胞产生氧气和释放钙。
DOI：
10.1182/blood.v68.2.535.535
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发表时间：
1986
期刊：
影响因子：
20.3
通讯作者：
R. Basford
中科院分区：
文献类型：
--
作者：
D. Kuhns;S. Kaplan;R. Basford
α-、γ-和δ-六氯环己烷（HCCH），而不是β-异构体，被证明是人体多形核白细胞（PMN）产生超氧阴离子（O2-）和释放钙的有效刺激物。虽然O2-生产发生在没有外源性二价阳离子，钙（0.5 mmol/L）提高O2-生产的50%。此外，γ-HCCH诱导的O2-产生对假定的细胞内拮抗剂8-（二乙基氨基）辛基-3，4，5-三甲氧基苯甲酸酯盐酸盐（TMB-8）的抑制敏感（IC 50 -30-40 mumol/L）。此外，它表明，γ-HCCH诱导膜相关钙的显着损失，监测金霉素荧光。该钙池似乎包含由甲酰化三肽甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）释放的相同钙池。我们认为，HCHs代表了一类新的刺激O2-生产和钙动员的中性粒细胞。
alpha-, gamma-, and delta-Hexachlorocyclohexane (HCCH), but not the beta-isomer, are shown to be potent stimuli for the production of superoxide anion (O2-) and the release of calcium in human polymorphonuclear leukocytes (PMNs). Although O2- production occurs in the absence of exogenous divalent cations, calcium (0.5 mmol/L) enhances O2- production by 50%. In addition, gamma-HCCH-induced O2- production is sensitive (IC50-30-40 mumol/L) to inhibition by the putative intracellular antagonist, 8-(diethylamino)octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8). Furthermore, it is shown that gamma-HCCH induces a marked loss of membrane-associated calcium as monitored by chlortetracycline fluorescence. This pool of calcium appears to encompass the same pool released by the formylated tripeptide, formylmethionylleucylphenylalanine (FMLP). We suggest that the HCCHs represent a new class of stimuli of O2- production and calcium mobilization in PMNs.