The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats
The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats
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DOI：
10.1267/ahc.19018
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发表时间：
2019-01-01
影响因子：
2.4
通讯作者：
Hishikawa, Yoshitaka
中科院分区：
文献类型：
--
作者：
Srisowanna, Naparee;Choijookhuu, Narantsog;Hishikawa, Yoshitaka
脂肪肝常见于男性和绝经后女性，提示雌激素可能参与肝脏脂质代谢。本研究的目的是明确雌激素和雌激素受体α（ER α）在肝再生过程中的作用，使用70%部分肝切除（PHX）模型，在雄性，雌性，卵巢切除（OVX）和E2治疗OVX（OVX-E-2）大鼠。在PHX后0-48 h采集肝组织，分别采用油红O、qRT-PCR和免疫组化检测脂肪堆积、脂肪酸移位酶（FAT/CD 36）、固醇调节元件结合蛋白（SREBP 1c）、过氧化物酶体增殖物激活受体α（PPAR α）、增殖细胞核抗原（PCNA）和ER α。与雄性和OVX大鼠相比，雌性和OVX-E-2大鼠的肝脏脂肪蓄积丰富。在PHX后0-12小时，在雌性、OVX和OVX-E-2中观察到FAT/CD 36表达，但在雄性大鼠中未观察到。在0小时，SREBP 1c和PPAR α分别在雌性和雄性大鼠中升高，但在所有大鼠中PHX后均降低。OVX-E-2和OVX大鼠PCNA标记指数分别在36小时和48小时达到最大值。在PHX后0-36小时，OVX-E-2中的ER α表达高于OVX。这些结果表明，雌激素和ER α可能在大鼠肝再生早期与FAT/CD 36相关的脂肪积累中起重要作用。
Fatty liver is common in men and post-menopausal women, suggesting that estrogen may be involved in liver lipid metabolism. The aim of this study is to be clear the role of estrogen and estrogen receptor alpha (ER alpha) in fat accumulation during liver regeneration using the 70% partial hepatectomy (PHX) model in male, female, ovariectomized (OVX) and E-2-treated OVX (OVX-E-2) rats. Liver tissues were sampled at 0-48 hr after PHX and fat accumulation, fatty acid translocase (FAT/CD36), sterol regulatory element-binding protein (SREBP1c), peroxisome proliferator-activated receptor alpha(PPAR alpha), proliferative cell nuclear antigen (PCNA) and ER alpha were examined by Oil Red O, qRT-PCR and immunohistochemistry, respectively. Hepatic fat accumulation was abundant in female and OVX-E-2 compared to male and OVX rats. FAT/CD36 expression was observed in female, OVX and OVX-E-2 at 0-12 hr after PHX, but not in male rats. At 0 hr, SREBP1c and PPAR alpha were elevated in female and male rats, respectively, but were decreased after PHX in all rats. The PCNA labeling index reached a maximum at 36 hr and 48 hr in OVX-E-2 and OVX rats, respectively. ER alpha expression in OVX-E-2 was higher than OVX at 0-36 hr after PHX. In conclusion, these results indicated that estrogen and ERa might play an important role in fat accumulation related to FAT/CD36 during early phase of rat liver regeneration.