H 2 Gene , nm 23 Identification of a Second Human Updated
H 2 Gene , nm 23 Identification of a Second Human Updated
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发表时间：
2006
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通讯作者：
J. Stahl;A. Leone;A. Rosengard;L. Porter;C. Richter King;Patricia S. Steeg
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作者：
J. Stahl;A. Leone;A. Rosengard;L. Porter;C. Richter King;Patricia S. Steeg
与鼠nm 231和人nm 231-III互补DNA克隆对应的RNA和/或蛋白质水平降低与几种啮齿动物模型系统和人乳腺癌中的高肿瘤转移潜力相关。我们报告的第二个人nm 23基因，命名为nm 23-H2的鉴定。pNM 23-H2S互补DNA克隆预测了与nm 23-H1 88%相同的M17，000蛋白。iin）23- 112也与核苷二磷酸激酶和果蝇发育基因具有显著的同源性。Southern印迹含有Bgt\I-限制性基因组DNA，其表现出nmII-III的等位基因限制性片段长度多态性。在与nm 23-H2探针再杂交时含有非等位基因条带。因此，nm 23-Hl和nm 23112是不同的基因。nm 23-H1和nm 23-H2特异性探针与乳腺肿瘤和细胞系的北方印迹杂交表明，在高转移潜能肿瘤细胞中，nm 23-H1表达的降低程度大于nm 23-H2。这些数据表明存在一个独立调控的nm 23基因家族。
Reduced RNA and/or protein levels corresponding to the murine nm231 and human nmì.ì-III complementary DNA clones have been correlated with high tumor metastatic potential in several rodent model systems and human breast carcinomas. We report the identification of a second human nm23 gene, designated nm23-H2. The pNM23-H2S complementary DNA clone predicted a M, 17,000 protein 88% identical to nm23-Hl. iin)23-112also shared a significant homology with nucleoside diphosphate kinases and a Drosophila developmental gene. Southern blots containing Bgt\I-restricted genomic DNA, which exhibited an allelic restriction fragment length polymorphism for nmìì-lll. contained nonallelic bands upon rehybridization to the nm23-H2 probe. Thus, nm23-Hl and nm23112 are distinct genes. Northern blot hybridization of nm23-Hland nm23-//2-specif1c probes to breast tumors and cell lines indicated that nm23-Hl expression was reduced in high metastatic potential tumor cells to a greater extent than nm23-H2. The data indicate the existence of a family of independently regulated nm23 genes.