Synchrotron multimodal imaging in a whole cell reveals lipid droplet core organization
Synchrotron multimodal imaging in a whole cell reveals lipid droplet core organization
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DOI：
10.1107/s1600577520003847
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发表时间：
2020-05-01
影响因子：
2.5
通讯作者：
Froissard, Marine
中科院分区：
文献类型：
--
作者：
Jamme, Frederic;Cinquin, Bertrand;Froissard, Marine
细胞的脂滴(LD)核心主要由中性脂类、三酰甘油和/或酯(SE)组成。这些脂质在核心内的结构仍在争论中。已观察到LDS内的脂质分离，但有时被认为是LD分离和化学固定的伪影。在其天然状态和未改变的细胞环境中进行LD成像似乎对于克服这些可能的技术缺陷至关重要。在此，提供了用于研究纤维素中天然LDS超微结构的成像技术，结果表明，LDS是有组织的结构。低温软X射线层析成像和深紫外光(DUV)透射率成像显示，在LD核心的外围有Se的分配。此外，活细胞上的DUV透过率和色氨酸/酪氨酸自动荧光成像被结合在一起，以获得关于细胞化学成分的补充信息。这种多模式方法为活细胞中的新的无标记细胞器成像技术铺平了道路。
A lipid droplet (LD) core of a cell consists mainly of neutral lipids, triacylglycerols and/or steryl esters (SEs). The structuration of these lipids inside the core is still under debate. Lipid segregation inside LDs has been observed but is sometimes suggested to be an artefact of LD isolation and chemical fixation. LD imaging in their native state and in unaltered cellular environments appears essential to overcome these possible technical pitfalls. Here, imaging techniques for ultrastructural study of native LDs in cellulo are provided and it is shown that LDs are organized structures. Cryo soft X-ray tomography and deep-ultraviolet (DUV) transmittance imaging are showing a partitioning of SEs at the periphery of the LD core. Furthermore, DUV transmittance and tryptophan/tyrosine auto-fluorescence imaging on living cells are combined to obtain complementary information on cell chemical contents. This multimodal approach paves the way for a new label-free organelle imaging technique in living cells.