SYCP3-like X-linked 2 is expressed in meiotic germ cells and interacts with synaptonemal complex central element protein 2 and histone acetyltransferase TIP60
SYCP3-like X-linked 2 is expressed in meiotic germ cells and interacts with synaptonemal complex central element protein 2 and histone acetyltransferase TIP60
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SYCP3 样 X-linked 2 在减数分裂生殖细胞中表达，并与联会复合体中心元件蛋白 2 和组蛋白乙酰转移酶 TIP60 相互作用。
DOI：
10.1016/j.gene.2013.06.033
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发表时间：
2013-09-15
期刊：
影响因子：
3.5
通讯作者：
Han, Chunsheng
中科院分区：
文献类型：
--
作者：
Shi, Yu-Qiang;Zhuang, Xin-Jie;Han, Chunsheng
减数分裂是二倍体生殖细胞产生单倍体配子的过程。一个关键的事件是突触复合体的形成。在粗成期，X和Y染色体的未配对区域形成了一个特殊的结构，XY小体，在这个结构中，基因表达大部分是沉默的。在本研究中，我们发现sycp3样X-linked 2 （SLX2, 1700013H16Rik）是XLR （X-linked Lymphocyte-Regulated）家族的新成员，在减数分裂生殖细胞中特异性表达。在精母细胞中，SLX2在瘦素前期、瘦素前期和zygotene期分布于生殖细胞核中，在粗素期局限于XY小体。这种定位变化与磷酸化组蛋白H2AX （γ H2AX）一致，这是一种众所周知的性别体成分。通过酵母双杂交筛选和共免疫沉淀实验，我们发现SLX2与突触复合体中心元件蛋白2 （SYCE2）和组蛋白乙酰转移酶TIP60相互作用，该酶与DNA损伤部位含磷酸h2ax的核小体的重塑有关。这些结果表明SLX2可能参与减数分裂过程中DNA重组、突触复合体的形成以及性别体的维持。(C) 2013 Elsevier B.V.版权所有
Meiosis is the process by which diploid germ cells produce haploid gametes. A key event is the formation of the synaptonemal complex. In the pachytene stage, the unpaired regions of X and Y chromosomes form a specialized structure, the XY body, within which gene expression is mostly silenced. In the present study, we showed that SYCP3-like X-linked 2 (SLX2, 1700013H16Rik), a novel member of XLR (X-linked Lymphocyte-Regulated) family, was specifically expressed in meiotic germ cells. In the spermatocyte SLX2 was distributed in the nucleus of germ cells at the preleptotene, leptotene and zygotene stages and is then restricted to the XY body at the pachytene stage. This localization change is coincident with that of phosphorylated histone H2AX (gamma H2AX), a well-known component of the sex body. Through yeast two-hybrid screening and coimmunoprecipitation assays, we demonstrated that SLX2 interacts with synaptonemal complex central element protein 2 (SYCE2), an important component of synaptonemal complex, and histone acetyltransferase TIP60, which has been implicated in remodeling phospho-H2AX-containing nucleosomes at sites of DNA damage. These results suggest that SLX2 might be involved in DNA recombination, synaptonemal complex formation as well as sex body maintenance during meiosis. (C) 2013 Elsevier B.V. All rights reserved.