A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions
A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions
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DOI：
10.1126/sciadv.aay9634
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发表时间：
2020-04-01
期刊：
影响因子：
13.6
通讯作者：
Guo, Lihua
中科院分区：
文献类型：
--
作者：
Li, Pengfei;Wang, Shuangchao;Guo, Lihua
在这里，我们描述了一个三方环状单链（SS）DNA真菌病毒，命名为禾谷镰刀菌gemytripvirus 1（FgGMTV 1）。FgGMTV 1的基因组包含三个环状ssDNA片段（DNA-A、DNA-B和DNA-C）。序列比对和系统发育分析表明，FgGMTV 1是嵌套在家庭基因型病毒。我们还构建了DNA真菌病毒的第一个感染性DNA克隆。我们的研究结果表明，DNA-A和DNA-B是相互依赖的复制，并与严重减少菌落生长和毒力低下。DNA-C依赖于DNA-A和DNA-B进行复制，并且对于异常真菌表型的恢复是必需的。DNA-C还增强了病毒DNA在感染真菌中的积累，并允许稳定的定殖和容易通过分生孢子传播。这是第一个从真菌中分离出的多分DNA病毒。我们的系统发育分析还表明，FgGMTV 1的多组分基因组可能是从一个古老的基因组病毒的单组分基因组进化而来的。
Here, we describe a tripartite circular single-stranded (ss) DNA mycovirus, named Fusarium graminearum gemytripvirus 1 (FgGMTV1). The genome of FgGMTV1 comprises three circular ssDNA segments (DNA-A, DNA-B, and DNA-C). Sequence alignments and phylogenetic analyses showed that FgGMTV1 is nested within the family Genomoviridae. We also constructed the first infectious DNA clones of a DNA mycovirus. Our results show that DNA-A and DNA-B are mutually interdependent for their replication and are associated with severely reduced colony growth and hypovirulence. DNA-C relies on DNA-A and DNA-B for replication and is necessary for the recovery of abnormal fungal phenotypes. DNA-C also enhances the accumulation of viral DNA in infected fungi and permits stable colonization and easy transmission via conidia. This is the first multipartite DNA virus isolated from a fungus. Our phylogenetic analyses also suggest that the multipartite genome of FgGMTV1 may have evolved from a monopartite genome of an ancient genomovirus.