Design and fabrication of cell alignment device based on electrolytically-generated air bubbles, and its practical realization using polystyrene microbeads
Design and fabrication of cell alignment device based on electrolytically-generated air bubbles, and its practical realization using polystyrene microbeads
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基于电解产生气泡的细胞排列装置的设计和制造及其使用聚苯乙烯微珠的实际实现
DOI：
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发表时间：
2009
期刊：
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通讯作者：
T. Hatsuzawa
中科院分区：
文献类型：
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作者：
T. Endo;K. Tsuji;Yasuhiro Tanaka;Y. Yanagida;T. Hatsuzawa
对于未来的医学诊断和药物筛选，基于微机电系统（MEMS）技术的芯片单细胞分析将是必不可少的。基于芯片的单细胞分析能够对大量样品细胞进行彻底分析，减少样品体积和分析时间，降低成本。然而，先前报道的基于芯片的单细胞分析具有细胞对准的一些缺点，例如麻烦的样品处理和长的细胞对准时间到芯片上。在这项研究中，提出了一种基于电产生的气泡的细胞对准方法和装置。为了实际实现，使用微珠进行细胞对齐。有了这项技术，基于芯片的单细胞分析将更加简化，适用于医疗和药物输送应用。
For future medical diagnostics and drug screening, chip-based single cell analysis based on a micro-electro-mechanical system (MEMS) technology will be essential. Chip-based single cell analysis enables to perform exhaustive analysis of huge numbers of sample cells, reducing sample volume and analysis time and lowering the costs. However, the previously reported chip-based single cell analysis has some disadvantages of cell alignment, such as troublesome sample handling and long cell alignment time onto the chip. In this study, a cell alignment method and device based on electrically-generated air bubbles is presented. For practical realization, the cell alignment was carried out using microbeads. With this technique, the chip-based single cell analysis will be more simplified for medical and drug delivery applications.