Enhancing electron transfer at a cytochrome c-immobilized microelectrode and macroelectrode.
Enhancing electron transfer at a cytochrome c-immobilized microelectrode and macroelectrode.
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增强细胞色素 c 固定微电极和大电极的电子转移。
DOI：
10.1021/la049942y
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Yau,Siu-Tung
中科院分区：
文献类型：
--
作者：
Strauss,Ela;Thomas,Bill;Yau,Siu-Tung
使用循环伏安法研究了固定在由不同平面的高度取向热解石墨组成的微电极和宏观电极（宏观电极）的裸露表面上的细胞色素的氧化还原反应。使用简单的掩蔽方法制造蛋白质固定微电极。对于大电极和微电极，固定化细胞色素的氧化还原反应需要通过将循环伏安法的电化学电位最大值增加到高正值来激活。可以使用两种方法增强该蛋白质电极系统的氧化还原电流。与由基底面组成的大电极相比，由边缘面组成的微电极上吸附的细胞色素的氧化和还原电流表现出异常增强。两种电极表面化学性质的差异导致电流异常。通过降低电位最小值可以选择性地增强大电极的氧化电流。
The redox reaction of cytochromecimmobilized on the bare surfaces of microelectrodes and macroscopic electrodes (macroelectrodes) composed of different planes of highly oriented pyrolytic graphite has been investigated using cyclic voltammetry. The protein-immobilized microelectrodes were fabricated using a simple masking method. For both macroelectrodes and microelectrodes, the redox reaction of immobilized cytochromecneeds to be activated by increasing the electrochemical potential maximum of cyclic voltammetry to a high positive value. The redox currents of this protein−electrode system can be enhanced using two approaches. The oxidation and reduction currents of cytochromecadsorbed on microelectrodes that are composed of the edge plane show an anomalous enhancement compared to those for macroelectrodes composed of the basal plane. The difference in the surface chemical properties of the two kinds of electrodes results in the current anomaly. The oxidation current of the macroelectrode can be selectively enhanced by decreasing the potential minimum.