The validity of the Aspergillus nidulans linkage map
The validity of the Aspergillus nidulans linkage map
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DOI：
10.1006/fgbi.1997.0984
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发表时间：
1997-06-01
影响因子：
3
通讯作者：
Clutterbuck, AJ
中科院分区：
文献类型：
--
作者：
Clutterbuck, AJ
构巢曲霉连锁图作为背景审查的粘粒交叉杂交和基因组测序的物理映射。基于DNA的方法依赖于邻近性，因此所产生的图谱仅与最弱的链接一样好，而添加到连锁图谱中的每个新标记都可以提供对其邻居位置的独立确认。对A.有丝分裂交换的分析提供了一个可靠的框架，在许多情况下，易位二体学的研究证实了这一点。在此框架的基础上，有一个由可靠的三点减数分裂定位和第二组不太精确的定位基因座连接的骨干基因座。其结果是一个地图与高度的自我一致性，虽然一些领域的不确定性或冲突也指出。(C)北京：科学出版社.
The Aspergillus nidulans linkage map is reviewed as a background to physical mapping by cosmid cross-hybridization and genome sequencing. DNA-based methods depend on contiguity, so that the resulting maps are only as good as the weakest link, whereas each new marker added to a linkage map can provide independent confirmation of the positions of its neighbors. For all eight chromosomes of A. nidulans a reliable framework has been provided by analysis of mitotic crossing over, in many cases substantiated by the study of translocation disomics. Building on this framework, there is a backbone of loci linked by reliable three-point meiotic mapping and a second set of less precisely mapped loci. The result is a map with a high degree of self-consistency although some areas of uncertainty or conflict are also noted. (C) 1997 Academic Press.