Index, comprehensive microsatellite, and unified linkage maps of human chromosome 14 with cytogenetic tie points and a telomere microsatellite marker.
Index, comprehensive microsatellite, and unified linkage maps of human chromosome 14 with cytogenetic tie points and a telomere microsatellite marker.
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带有细胞遗传学连接点和端粒微卫星标记的人类 14 号染色体的索引、综合微卫星和统一连锁图。
DOI：
10.1006/geno.1995.9953
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Donis-Keller,H
中科院分区：
文献类型：
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作者：
Pandit,SD;Wang,JC;Veile,RA;Mishra,SK;Warlick,CA;Donis-Keller,H
三套连锁图谱（索引，综合微卫星，和统一）已被构建为人类14号染色体的基因型的基础上，从CEPH参考家系。索引图由18个微卫星标记组成，杂合度至少为68%，标记间间距不大于11 cM。性别平均综合微卫星图谱长度为125 cM，包括115个标记，其中54个位点独特地放置在标记顺序的赔率至少为1000：1。性别平均索引图长度为121 cM，女性和男性特有的地图分别为143和101 cM。利用147个位点（162个标记系统）构建了一个统一的图谱，其中包括32个RFLP标记和115个微卫星标记。该图的性别平均长度为128 cM，有69个位点是唯一定位的。我们的地图锚定的微卫星端粒标记sCAW 1（D14S826），从端粒YAC克隆TYAC 196，延伸的连锁图谱的物理末端的染色体14的长臂。此外，我们还物理映射的7个位点的粘粒克隆或荧光原位杂交扩增的PCR产物从YAC含有标记序列。与以前建立的其他基因座的细胞遗传学地图指定一起，我们的地图显示14号染色体的13个细胞遗传学带中的10个在550基因组带分辨率的遗传标记之间的联系。
Three sets of linkage maps (index, comprehensive microsatellite, and unified) have been constructed for human chromosome 14 based on genotypes from the CEPH reference pedigrees. The index maps consist of 18 microsatellite markers, with heterozygosities of at least 68% and intermarker spacing no greater than 11 cM. The sex-average comprehensive microsatellite map is 125 cM in length and includes 115 markers with 54 loci uniquely placed with odds for marker order of at least 1000:1. The sex-average index map length is 121 cM, and the female- and male-specific maps are 143 and 101 cM, respectively. A unified map was also constructed from 147 loci (162 marker systems), which includes 32 RFLP markers in addition to the 115 microsatellites. The sex-average length of the unified map is 128 cM with 69 loci uniquely placed. Our maps are anchored by a microsatellite telomere marker sCAW1 (D14S826), developed from a telomere YAC clone TYAC196, which extends the linkage map to the physical terminus of the long arm of chromosome 14. Furthermore, we have also physically mapped seven of the loci by fluorescencein situhybridization of cosmid clones orAlu-PCR products amplified from YACs containing the marker sequences. Together with previously established cytogenetic map designations for other loci, our maps display links between genetic markers for 10 of 13 cytogenetic bands of chromosome 14 at the 550 genome band resolution.