Dissecting Kinase Effector Signaling Using the RapRTAP Methodology.
Dissecting Kinase Effector Signaling Using the RapRTAP Methodology.
复制标题
使用 RapRTAP 方法剖析激酶效应器信号传导。
DOI：
10.1007/978-1-4939-7154-1_2
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Karginov,AndreiV
中科院分区：
文献类型：
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作者：
Ray,Anne-Marie;Klomp,JenniferE;Collins,KerrieB;Karginov,AndreiV
Kinases are involved in a broad spectrum of cell behaviors. A single kinase can interact with different ligands each eliciting a specific cellular response. Dissecting downstream signaling pathways of kinases is a key step to understanding physiological and pathological cell process. However, directing kinase activity to specific substrates remains challenging. Here, we present a new tool to selectively activate a kinase in a specific protein complex in living cells. This technology uses a rapamycin-inducible kinase activation coupled to interaction with FKBP12-binding domain (FRB) tagged protein. Here, we demonstrate application of this method by targeting Src to either p130Cas or FAK and discriminating cell mophodynamic changes downstream each of these signaling complexes.
