ORIGIN OF OSTEOCLASTS - MATURE MONOCYTES AND MACROPHAGES ARE CAPABLE OF DIFFERENTIATING INTO OSTEOCLASTS UNDER A SUITABLE MICROENVIRONMENT PREPARED BY BONE MARROW-DERIVED STROMAL CELLS
ORIGIN OF OSTEOCLASTS - MATURE MONOCYTES AND MACROPHAGES ARE CAPABLE OF DIFFERENTIATING INTO OSTEOCLASTS UNDER A SUITABLE MICROENVIRONMENT PREPARED BY BONE MARROW-DERIVED STROMAL CELLS
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DOI：
10.1073/pnas.87.18.7260
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发表时间：
1990-09-01
影响因子：
11.1
通讯作者：
SUDA, T
中科院分区：
文献类型：
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作者：
UDAGAWA, N;TAKAHASHI, N;SUDA, T
我们先前报道了在1 α，25-二羟维生素D3和地塞米松。在这项研究中，我们开发了一种新的共培养系统，以确定破骨细胞的起源。当从小鼠骨髓、脾、胸腺或外周血获得的相对少量的单核细胞（每孔103-105个细胞）在ST 2细胞层上培养12天时，它们形成集落，所形成的集落数与接种的造血细胞数之间具有线性关系。抗酒石酸酸性磷酸盐（TRAPase）阳性单核细胞和多核细胞出现在响应1 α的集落（TRAPase-阳性集落）中，25-二羟维生素D3和地塞米松。当在ST 2细胞层上培养悬浮在胶原凝胶溶液中的造血细胞以防止它们移动时，类似地形成TRAPases阳性集落，表明每个集落源自单个细胞。所有集落均由非特异性酯酶阳性细胞组成。从外周血制备的单核细胞耗尽的人口未能形成集落，而单核细胞富集的人口产生了大量的TRAPase-positive菌落。此外，肺泡巨噬细胞在两种激素的存在下最有效地在ST 2细胞层上形成TRAP阳性集落。鲑鱼125 I标记的降钙素特异性结合TRAPase阳性细胞。在牙本质切片上形成吸收陷窝，进行共培养。当外周血细胞和ST 2细胞之间的直接接触被胶原凝胶片抑制时，没有TRAPase阳性细胞形成。这些结果表明，破骨细胞也来自成熟的单核细胞和巨噬细胞时，骨髓来源的基质细胞提供了一个合适的微环境。
We previously reported that osteoclast-like cells were formed in cocultures of a mouse marrow-derived stromal cell line (ST2) with mouse spleen cells in the presence of 1.alpha.,25-dihydroxyvitamin D3 and dexamethasone. In this study, we developed a new coculture system to determine the origin of osteoclasts. When relatively small numbers of mononuclear cells (103-105 cells per well) obtained from mouse bone marrow, spleen, thymus, or peripheral blood were cultured for 12 days on the ST2 cell layers, they formed colonies with a linear relationship between the number of colonies formed and the number of hemopoietic cells inoculated. Tartrate-resistant acid phosphate (TRAPase)-positive mononuclear and multinucleated cells appeared in the colonies (TRAPase-positive colonies) in response to 1.alpha.,25-dihydroxyvitamin D3 and dexamethasone. When hemopoietic cells suspended in a collagen-gel solution were cultured on the ST2 cell layers to prevent their movement, TRAPase-positive colonies were similarly formed, indicating that each colony originated from a single cell. All of the colonies consisted of nonspecific esterase-positive cells. The monocyte-depleted population prepared from peripheral blood failed to form colonies, whereas the monocyte-enriched population produced a larger number of TRAPase-positive colonies. In addition, alveolar macrophages formed TRAP-positive colonies most efficiently on the ST2 cell layers in the presence of two hormones. Salmon 125I-labeled calcitonin specifically bound to the TRAPase-positive cells. Resorption lacunae were formed to dentine slices on which cocultures were performed. When direct contact between the peripheral blood cells and the ST2 cells was inhibited by a collagen-gel sheet, no TRAPase-positive cells were formed. These results indicate that osteoclasts are also derived from the mature monocytes and macrophages when a suitable microenvironment is provided by bone marrow-derived stromal cells.