GABA as a trophic factor during development.
GABA as a trophic factor during development.
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GABA 作为发育过程中的营养因子。
DOI：
10.1016/0024-3205(83)90754-3
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发表时间：
1983
期刊：
影响因子：
6.1
通讯作者：
Redburn,DA
中科院分区：
文献类型：
--
作者：
MadtesJr,P;Redburn,DA
许多研究者已经研究了突触发生的过程，以确定调节突触形成的因素。本研究利用新生兔视网膜研究了γ-氨基丁酸（GABA）神经递质系统在视网膜发育过程中的作用。通过利用离体眼杯的体外处理，我们发现用GABA摄取阻断剂哌啶甲酸处理导致特异性3 H-蝇蕈醇结合量增加4倍。此外，在GABA激动剂蝇蕈醇、4，5，6，7-四氢异恶唑并[5，4-c]吡啶-3-醇（THIP）或GABA本身存在下孵育组织导致特异性3 H-蝇蕈醇结合的类似增加。这些发现支持了先前研究的结论，即用哌啶甲酸体内处理1日龄家兔后观察到的GABA受体诱导是由于GABA的细胞外浓度增加。GABA在GABA能突触形成的调节中可能起作用。
The process of synaptogenesis has been studied by many investigators to determine the factors which regulate synapse formation. We have used neonatal rabbit retina to investigate the role of the γ-aminobutyric acid (GABA) neurotransmitter system during development. By utilizing an in vitro in treatment of isolated eyecups we found that treatment with nipecotic acid, a GABA uptake blocker, resulted in a 4-fold increase in the amount of specific 3 H-muscimol binding. In addition, incubation of the tissue in the presence of the GABA agonists muscimol, 4, 5, 6, 7-tetrahydroisoxazolo [5, 4-c] pyridine-3-ol (THIP), or GABA itself led to similar increases in specific 3 H-muscimol binding. The findings support the conclusion from previous studies that the induction of GABA receptors observed after in vivo treatment of 1-day-old rabbits with nipecotic acid resulted from an increase in the extracellular concentration of GABA. A possible role for GABA in the regulation of GABAergic synapse formation is presented in this report.